[PDF] Top 20 The Genome Sequence of Herpes Simplex Virus Type 2

... Our evaluation of the presence of protein-coding regions primarily involved criteria based on characteristics of DNA and protein sequences, as distinct from experimental observa- tion of transcripts and proteins or from ... See full document

... The evidence was as follows: i HSV-2-transformed cells and the derived tumor cells gave a positive reaction with HSV-1 antiserum, but other DNA virus-induced tumor cells did not; and ii [r] ... See full document

... 420-429 0022-538X/81/050420-10$02.00/0 Mechanisms of Expression of Herpes Simplex Virus-Common Surface Antigens in Clonal Cells of a Herpes Simplex Virus Type 2-Transformed Line KATSUICH[r] ... See full document

... Vials containing a disc with I ,ug of HSV-DNA and a blank disc were preincubated with: i unlabeled nuclear RNA isolated at 6.5 hr after infection from infected cells incubated with 2.5 m[r] ... See full document

... entire genome, a fragment was randomly selected from the HSV-1 genome, in which repeats were painted in differ- ent color depending on difference of iterations (Figure ...the genome and presented a ... See full document

... Analysis of origin sequences in herpes simplex virus type 2 HSV-2 has shown that deletion of part of a sequence corresponding to site III in HSV-1 resulted in a dramatic loss in DNA repl[r] ... See full document

... A separate primer and probe mix are supplied with this kit to detect the exogenous DNA using real-time PCR. The primers are present at PCR limiting concentrations which allows multiplexing with the target sequence ... See full document

... Effect of endo H on newly synthesized and immunoprecipitation with anti-gF-2 111188 processed forms of gF-2 immunoprecipitated monoclonal antibody followed by preparative anti-gF-2 III18[r] ... See full document

... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document

... cells (34% neutrophils, 27% lymphocytes, 39% mono- macrophages), and glucose and protein concentrations of 3.6 mmol/L and 2.1 g/L, respectively. A polymerase chain reaction (PCR) of the CSF detected HSV type ... See full document

... For long-term lahave examined the polypeptides precipitated by beling of infected cell peptides 1 to 16 h or for shortmonoclonal antibodies secreted by two of the time pulsing 2-h period[r] ... See full document

... In addi- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We used monoclonal antibodies reacting with glycoproteins specified by herpes simplex virus type 2 HSV-2 to cha[r] ... See full document

... not virus shedding after intraperitoneal inoculation in mice (51) (presumably related to vhs-mediated DC inactivation [83]; however, the mutant retained virulence ... See full document

... TEVETHIA* Department of Microbiology and Cancer Research Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 A functions involved in the altered su[r] ... See full document

... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document

... FIG 3 Inhibition of DNA replication by the AAV2 Rep helicase activity. (A) Schematic representation of the Rep constructs analyzed in this experiment. The functional protein domains of interest for this study are ... See full document

... ChIP and seq-ChIP. ChIP was performed as described previously (20). In summary, confluent plates of HeLa cells were infected in the absence or presence of actinomycin D or cycloheximide, as indicated in the figure ... See full document

... Genetic analysis of temperature-sensitive mutants which define the genes for the major herpes simplex virus type 2 DNAbinding protein and a new late function.. Studies of defective herpe[r] ... See full document

... The first evidence that the UL25 gene product was impor- tant for DNA packaging was obtained from the analysis of two HSV-1 mutants, ts1204 and ts1208, that had temperature-sen- sitive (ts) mutations in the UL25 gene ... See full document

... Because nearly all of the 34 new genomes were from low-pas- sage-number isolates, we compared the nucleotide and amino acid divergences of their ORFs from those of the HG52 laboratory strain (both the Sanger RefSeq ... See full document