[PDF] Top 20 Mapping of the Hepatitis B Virus Reverse Transcriptase TP and RT Domains by Transcomplementation for Nucleotide Priming and by Protein-Protein Interaction
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Mapping of the Hepatitis B Virus Reverse Transcriptase TP and RT Domains by Transcomplementation for Nucleotide Priming and by Protein-Protein Interaction
... the TP and RT domains were created by PCR ...both TP and RT, the forward primer fused the FLAG epitope to the HBV sequence starting at the first amino acid number designated in the ... See full document
9
Hepatitis B Virus Reverse Transcriptase and ε RNA Sequences Required for Specific Interaction In Vitro
... HBV RT-ε interaction directly, we have recently developed an in vitro reconstitution system for HBV RNP formation, using purified HBV RT proteins expressed in bacteria and components of the Hsp90 ... See full document
10
Requirement of Heat Shock Protein 90 for Human Hepatitis B Virus Reverse Transcriptase Function
... HBV RT- interaction in vitro by using purified RT and Hsp90 chaperone ...HBV RT-ε interaction, it was conceivable that Hsp90 was required for pgRNA packaging through a mechanism ... See full document
10
Mapping of Functional Subdomains in the Terminal Protein Domain of Hepatitis B Virus Polymerase
... ABSTRACT Hepatitis B virus (HBV) encodes a multifunction reverse transcriptase or polymerase (P), which is composed of several ...terminal protein (TP) do- main is unique ... See full document
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TP-RT Domain Interactions of Duck Hepatitis B Virus Reverse Transcriptase in cis and in trans during Protein-Primed Initiation of DNA Synthesis In Vitro
... minimal RT domain sequence required for trans-comple- mentation was also further narrowed down to position 366 to 555 ...full-length RT protein trans- lated in RRL, abolished protein ... See full document
15
A Single Amino Acid in the Reverse Transcriptase Domain of Hepatitis B Virus Affects Virus Replication Efficiency
... Hepatitis B virus (HBV) infection results in a broad spec- trum of clinical manifestations, including asymptomatic car- riage, acute self-limited hepatitis, chronic hepatitis, and ... See full document
7
In Vitro Reconstitution of a Functional Duck Hepatitis B Virus Reverse Transcriptase: Posttranslational Activation by Hsp90
... Hepatitis B virus (HBV) infection is a major public health problem, with over 300 million chronically infected people worldwide ...by reverse transcription carried out by a virally encoded ... See full document
9
Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cells.
... terminal protein (TP), RT, and RNase H domains are indicated above the polymerase open reading ...a nucleotide priming reaction templated by the bulge in epsilon, and then the ... See full document
9
Cryptic Protein Priming Sites in Two Different Domains of Duck Hepatitis B Virus Reverse Transcriptase for Initiating DNA Synthesis In Vitro
... cryptic priming sites on RT also sug- gests that RT may be able to use other proteins, viral or host, as primers to initiate DNA ...the protein primer VPg, which is not covalently linked to ... See full document
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Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.
... at nucleotide (nt) 2309 ...a virus encoding the first 334 amino acids of pol with nine additional non-pol amino acids and still containing all downstream HBV RNA sequences, including DR1 and the epsilon ... See full document
9
Interaction between Hepatitis B Virus Core Protein and Reverse Transcriptase
... with hepatitis B virus (HBV) suggest that continued interactions with core are required for several steps in genomic ...terminal protein (TP), reverse transcriptase ... See full document
11
Reverse Transcriptase- and RNA Packaging Signal-Dependent Incorporation of APOBEC3G into Hepatitis B Virus Nucleocapsids
... deaminase protein A3G into HBV ...the protein, with the exception of A3G 104-384. (B and C) HepG2 cells were transfected with a plasmid expressing either the full-length A3G or the A3G deletion ... See full document
10
Interaction between hepatitis C virus core protein and E1 envelope protein.
... C virus has three structural genes named C, E1, and ...(capsid) protein and the E1 and E2 genes encode the envelope ...E1 protein was found to be precipitated by an anti-core antibody in the presence ... See full document
6
The Majority of Duck Hepatitis B Virus Reverse Transcriptase in Cells Is Nonencapsidated and Is Bound to a Cytoplasmic Structure
... core protein (data not shown), eliminating the possibility that polymerase detected by immunoprecipitation was derived from immature cores that were detergent sensi- ...core protein versus polymerase not ... See full document
10
Interaction of Reverse Transcriptase (RT) Mutations Conferring Resistance to Lamivudine and Etravirine: Effects on Fitness and RT Activity of Human Immunodeficiency Virus Type 1
... real-time reverse transcriptase-coupled PCR (RT-PCR) with an ABI PRISM 7300 sequence detection system (Applied Biosystems, ...initial reverse transcription reac- tion was performed at 50°C for ... See full document
6
Reconstitution of a Functional Duck Hepatitis B Virus Replication Initiation Complex from Separate Reverse Transcriptase Domains Expressed in Escherichia coli
... P protein open reading frame (ORF) plus the 3⬘ copy of Dε. The TP and RT constructs were obtained by appropriate restriction digestions and/or PCR-mediated mutagenesis (details are available from the ... See full document
10
Protein-x of hepatitis B virus in interaction with CCAAT/enhancer-binding protein α (C/EBPα) - an in silico analysis approach
... and protein-x, compara- tive models were not ...initio protein folding and protein structure prediction. Protein-x is a relatively small protein, therefore prediction of the tertiary ... See full document
10
Sendai virus protein-protein interactions studied by a protein-blotting protein-overlay technique: mapping of domains on NP protein required for binding to P protein.
... In an attempt to understand which component of the nucleocapsid mediates binding of P protein, we separated Sendai virus proteins by SDS-PAGE, blotted them on PVDF membranes, renatured t[r] ... See full document
6
Accumulation of mutations in reverse transcriptase of hepatitis B virus is associated with liver disease severity in treatment-naïve Chinese patients with chronic hepatitis B
... these RT mutations were selected under liver in- flammation induced by the virus and host immune re- sponse, we compared the clinical data of patients with and without RT mutations and found that no ... See full document
7
cis rescue of a mutated reverse transcriptase gene of human hepatitis B virus by creation of an internal ATG.
... This difference between wild-type and mutant 2306 cannot be attributed to a concurrent structural alteration of the core protein by mutation 2306, which converts a proline residue into a[r] ... See full document
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