[PDF] Top 20 Physical mapping of herpes simplex virus type 1 mutations by marker rescue.
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Physical mapping of herpes simplex virus type 1 mutations by marker rescue.
... We have screened separated restriction endonuclease fragments of WT DNA for their ability to rescue progeny virus from cells mixedly infected at nonpermissive temperature with intact DNA[r] ... See full document
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Physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue.
... Figure 1 shows the physical maps of HSV-1 strain 17 and HSV-2 strain HG52 for the restriction endonucleases XbaI, HindIII, EcoRI, BglII, HpaI, KpnI, and BamHI in the region 30 to 50 map [r] ... See full document
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Exonuclease-Deficient Polymerase Mutant of Herpes Simplex Virus Type 1 Induces Altered Spectra of Mutations
... To marker rescue the pol mutation within recombinant Y7/F-1, Vero cells were cotransfected with Y7/F-1 infectious virus DNA and different pol DNA fragments by using ...to rescue ... See full document
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Generation of Herpesvirus Entry Mediator (HVEM)-Restricted Herpes Simplex Virus Type 1 Mutant Viruses: Resistance of HVEM-Expressing Cells and Identification of Mutations That Rescue Nectin-1 Recognition
... viously identified in HVEM cDNAs isolated from chronic lym- phocytic leukemic B cells (14) and two HSV-seronegative in- dividuals (46). Struyf and coworkers demonstrated that V241I does not alter the HSV entry-receptor ... See full document
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Physical mapping of two herpes simplex virus type 1 host shutoff loci: rescue of each ts mutation occurs with two unique cloned regions of the viral genome.
... 143 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Two complementing temperature-sensitive ts herpes simplex virus type 1 HSV-1 mutants, PAAirtsl and ts199, were defec[r] ... See full document
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Functional and Physical Interactions of the Herpes Simplex Virus Type 1 UL20 Membrane Protein with Glycoprotein K
... UL20-null virus, which contains an EGFP gene cassette within the UL20p open reading ...to rescue the UL20 deletion and transfer the UL20p genes with their 3 ⫻ FLAG epitope tags into the ... See full document
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Fine-structure mapping of herpes simplex virus type 1 temperature-sensitive mutations within the short repeat region of the genome.
... PRESTON Medical Research Council Virology Unit, University of Glasgow, Glasgow Gll 5JR, Scotland Cloned herpes simplex virus type 1 HSV-1 DNA fragments were used to finestructure map the[r] ... See full document
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Phosphorylation Site Mutations Affect Herpes Simplex Virus Type 1 ICP0 Function
... The herpes simplex virus type 1 (HSV-1) immediate-early (IE) regulatory protein infected-cell protein 0 (ICP0) is a strong and global transactivator of both viral and cellular ... See full document
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Physical mapping and nucleotide sequence of a herpes simplex virus type 1 gene required for capsid assembly.
... 2169 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 H[r] ... See full document
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Mode of Virus Rescue Determines the Acquisition of VHS Mutations in VP22-Negative Herpes Simplex Virus 1
... 22 virus which had been constructed by classical homologous recombination in the background of the strain 17 (s17) genome ...This virus was originally recovered from a VP22 complementing cell line, but when ... See full document
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Physical mapping of the herpes simplex virus type 2 nuc- lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones.
... * 237 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The nuc- lesion affecting alkaline exonuclease activity in the herpes simplex virus type 2 HSV-2 mutant ts1348 had[r] ... See full document
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Fine mapping and molecular cloning of mutations in the herpes simplex virus DNA polymerase locus.
... 236 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Mutations in five phenotypically distinct mutants derived from herpes simplex virus type 1 strain KOS which lie in o[r] ... See full document
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Mapping of a herpes simplex virus type 2-encoded function that affects the susceptibility of herpes simplex virus-infected target cells to lysis by herpes simplex virus-specific cytotoxic T lymphocytes.
... TEVETHIA* Department of Microbiology and Cancer Research Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 A functions involved in the altered su[r] ... See full document
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Syncytial mutations in the herpes simplex virus type 1 gK (UL53) gene occur in two distinct domains.
... The fact that all of the mutants sequenced had mutations resulting in amino acid substitutions in the gK gene strengthens the identification of this gene with the synl locus.. The fragme[r] ... See full document
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Ribonucleotides Linked to DNA of Herpes Simplex Virus Type 1
... It should be added that RNase treatment of a comparable sample of heat-denatured HSV DNA which had been purified in cesium sulfate gradient resulted in a significant decrease of [3Hjurid[r] ... See full document
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Isolation and localization of herpes simplex virus type 1 mRNA.
... Finally, vii the 4-kb species, as well as a range of smaller species of viral RNA species found hybridizing to HindIII fragment G cellulose and blots, are shown in the short unique regio[r] ... See full document
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Herpes simplex virus type 1-induced hydrocephalus in mice.
... When the cranial cavities of these mice were opened, hydrocephali had developed in the group of mice that received whole immune spleen cells and those treated with anti-immunoglobulin pl[r] ... See full document
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Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome.
... We isolated specific HSV-1 mRNA species mapping within the region of interest by hybridization of late polyA mRNA to the appropriate restriction fragment bound to cellulose see above.. S[r] ... See full document
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Identification of a promoter mapping within the reiterated sequences that flank the herpes simplex virus type 1 UL region.
... GELMAN, AND SAUL SILVERSTEIN* Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032 Analysis of the promoter for the herpes simple[r] ... See full document
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Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
... Mass spectrometry. Purified virions were first acetone precipitated and solu- bilized in Laemmli buffer (2% SDS, 10% glycerol, 5% -mercaptoethanol, 0.002% bromophenol blue, and 62.5 mM Tris-HCl [pH 6.8]). Twenty ... See full document
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