[PDF] Top 20 Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression.
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Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression.
... The patterns of viral protein synthesis at 3 or 6 h postinfection were similar in the 3 different cell lines, except that significant amounts of the mutant form of ICP8 expressed from th[r] ... See full document
10
Herpes Simplex Virus Type 1 Origins of DNA Replication Play No Role in the Regulation of Flanking Promoters
... Herpes simplex virus (HSV) gene expression during the course of in vitro infection has been well characterized (5, 6, 20, ...tegument protein virion protein 16 (VP16) and ... See full document
10
ICP27 Phosphorylation Site Mutants Are Defective in Herpes Simplex Virus 1 Replication and Gene Expression
... Herpes simplex virus 1 (HSV-1) protein ICP27 is a multifunctional regulatory protein that is posttransla- tionally modified by phosphorylation during viral ...a protein kinase A ... See full document
12
Elimination of Mitochondrial DNA Is Not Required for Herpes Simplex Virus 1 Replication
... AN1 virus infection (43) and demonstrate that UL98 can restore viral repli- cation to near wild-type levels in the absence of UL12 and ...Viral protein expression. Since the KOS37 UL98 virus ... See full document
10
Role of the Herpes Simplex Virus Helicase-Primase Complex during Adeno-Associated Virus DNA Replication
... helper protein assay, with some mutants displaying significant impairments ...AAV DNA replication but retained the ability to mediate nuclear colocalization of ICP8 and Rep ...AAV DNA, ... See full document
10
Origin Binding Protein-Containing Protein-DNA Complex Formation at Herpes Simplex Virus Type 1 oriS: Role in oriS-Dependent DNA Replication
... UL8.5 gene, is the product of a unique delayed-early transcript that originates within the open reading frame (ORF) of the gene encoding OBP (UL9) ...the DNA polymerase processivity factor ...origin ... See full document
9
Pocket Protein p130/Rb2 Is Required for Efficient Herpes Simplex Virus Type 1 Gene Expression and Viral Replication
... viral DNA synthesis pro- ceeds, the staining pattern evolves to a globular appearance as replication compartments begin to fill the nucleus (15, 48, ...nuclear ICP8 staining pattern was observed in ... See full document
12
The Ability of Herpes Simplex Virus Type 1 Immediate-Early Protein Vmw110 To Bind to a Ubiquitin-Specific Protease Contributes to Its Roles in the Activation of Gene Expression and Stimulation of Virus Replication
... a role in the control of protein stability since they cleave ubiquitin adducts from substrate proteins, thereby protecting the substrate from proteasome-mediated ...of gene expression mediated ... See full document
10
Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... UL25 protein is one of seven viral proteins that are required for DNA cleavage and ...of DNA-containing C capsids on the capsid ...multifunctional protein required: (i) to stabilize capsids ... See full document
11
The Initiator Element in a Herpes Simplex Virus Type 1 Late-Gene Promoter Enhances Activation by ICP4, Resulting in Abundant Late-Gene Expression
... explain late- gene expression during viral ...viral replication, and differences in polyadenylation sites (53, 62, 76, ...in late genes play a role in temporal ...in ... See full document
11
Role of Cellular Phosphatase cdc25C in Herpes Simplex Virus 1 Replication
... viral DNA during HSV-1(F) infection was lower in cdc25C ⴚ / ⴚ cells than in cdc25C ⴙ / ⴙ MEF ...a role in HSV-1 DNA ...a role in viral DNA accumulation, total DNA was purified ... See full document
6
Retention of the herpes simplex virus type 1 (HSV-1) UL37 protein on single-stranded DNA columns requires the HSV-1 ICP8 protein.
... Analysis of extracts derived from cells coinfected with V37 and an ICP8-expressing vaccinia virus recombinant V8 and analysis of extracts prepared from cells infected with the HSV-1 ICP8[r] ... See full document
5
The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication.
... It contains two host range alterations in the amino-terminal portion of the gene the Ad2hr400 mutation and the cold-resistant mutation that allow the virus to overcome the normal block t[r] ... See full document
15
Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
10
Herpes simplex virus type 1 ICP8: helix-destabilizing properties.
... The assay was performed in the presence of M13-22-mer substrate, 0.27 ,uM ICP8, and the indicated concentrations of MgCI2 as described in Materials and Methods.. Kinetics of strand displ[r] ... See full document
5
Mutations in the major DNA-binding protein gene of herpes simplex virus type 1 result in increased levels of viral gene expression.
... We have found that at the nonpermissive temperature, the synthesis of certain immediate early, early, and late viral polypeptides was greater in cells infected with the temperature-sensi[r] ... See full document
9
Herpes simplex virus DNA synthesis at a preformed replication fork in vitro.
... 4957 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Proteins from herpes simplex virus HSV-infected cells were used to reconstitute DNA synthesis in vitro on preformed[r] ... See full document
11
The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation
... with 70% ethanol, air dried briefly, and resuspended in sample buffer (10 mM morpholinepropanesulfonic acid [MOPS; pH 7.0], 4 mM sodium acetate, 0.5 mM EDTA, 50% freshly thawed formamide, 6% formaldehyde, 7% glycerol). ... See full document
6
Mapping of the transcriptional initiation site of the herpes simplex virus type 1 ICP8 gene in infected and transfected cells.
... 615 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The initiation site for transcription of the herpes simplex virus type 1 HSV-1 gene encoding the major DNA-binding p[r] ... See full document
6
A physical domain of herpes simplex virus ICP8 is expressed and active in Escherichia coli.
... coli containing ICP8-active plasmids Plasmid or plasmid insert pBR322 BamHI-G G-V site near tet promoter BamHI-G inverted BamHI-SalI-GO AO BamHI-G [from HSV-1mP tsHA1] New BamHI-G-contai[r] ... See full document
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