[PDF] Top 20 Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... III protein and a random pentapeptide fused to human growth ...fusion protein is synthesized and anchored on the surface of phage particle in the presence of helper ... See full document
9
Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27.
... 1987 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Varicella-zoster virus VZV open reading frame 4 ORF4 encodes a putative immediate-early protein which is homologous [r] ... See full document
6
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene.
... in frame of the oligonucleotide created by hybridization of the single-stranded oligonucleotides 5 9 -CTGACGAGTACGACGACGCAGCC GACGCCGCCGGCGACCGGGCCCCGAAGCTTG-3 9 and 5 9 -CAAGCTT ... See full document
7
The Herpes Simplex Virus Type 1 UL3 Transcript Starts within the UL3 Open Reading Frame and Encodes a 224-Amino-Acid Protein
... the protein-antibody complex ...FIG. 1. (A) Schematic diagram of the HSV-1 genome illustrating the relative sizes and locations of HSV-1 UL3-specific transcripts in the UL1-UL3 ...line ... See full document
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The transcriptional activation domain of varicella-zoster virus open reading frame 62 protein is not conserved with its herpes simplex virus homolog.
... Plasmid GALA contains the DNA binding domain amino acids 1 to 147 [18] of the yeast GAL4 protein preceded by the simian virus 40 early promoter and followed by a polylinker sequence 29..[r] ... See full document
6
Varicella-zoster virus (VZV) open reading frame 10 protein, the homolog of the essential herpes simplex virus protein VP16, is dispensable for VZV replication in vitro.
... To determine whether substitution of VZV ORF10 by HSV-1 VP16 alters the growth of the virus in cell culture, we inoculated melanoma cells with cells infected with VZV ROka, ROkalOD/VP16A[r] ... See full document
9
The Capsid Protein Encoded by UL17 of Herpes Simplex Virus 1 Interacts with Tegument Protein VP13/14
... ⫹ 1 insertion mutation in HSV-1(F) U L 17 was predicted to cause a frame- shift relative to HSV-1(17) at codon 11 ...The open reading frames then realign at codon 21 by a ... See full document
9
The unique sequence of the herpes simplex virus 1 L component contains an additional translated open reading frame designated UL49.5.
... This gene, consisting of the authentic promoter and chimeric coding domain, was inserted into the thymidine kinase gene of wild-type virus and in infected cells expressed a protein which[r] ... See full document
5
Transcription of the derepressed open reading frame P of herpes simplex virus 1 precludes the expression of the antisense gamma(1)34.5 gene and may account for the attenuation of the mutant virus.
... tory protein, infected cell protein 4 (ICP4), is dysfunctional or ...to 1 3) ...expressed open reading frames mapping within the domain of the viral genome tran- scribed during ...16 ... See full document
8
Prediction and Identification of Herpes Simplex Virus 1-Encoded MicroRNAs
... At present, there are few genetic clues for biological activ- ities of HSV miRNAs. The DNA sequence encoding HSV-1 pre-miR-H1 spans the 5 ⬘ untranslated region and the open reading frame of ... See full document
10
Varicella-zoster virus open reading frame 10 protein, the herpes simplex virus VP16 homolog, transactivates herpesvirus immediate-early gene promoters.
... Furthermore, cell lines stably expressing ORF10 complement the HSV-1 mutant in1814, which lacks the transactivating function of VP16, and enhance the de novo synthesis of infectious viru[r] ... See full document
8
Cell lines containing varicella-zoster virus open reading frame 62 and expressing the "IE" 175 protein complement ICP4 mutants of herpes simplex virus type 1.
... Three neomycin-resistant cell lines were isolated and shown to complement two different ICP4 mutants tsB21 and d120 of herpes simplex virus HSV type 1 HSV-1.. VZV-specific RNA could not [r] ... See full document
7
Up to Four Distinct Polypeptides Are Produced from the γ34.5 Open Reading Frame of Herpes Simplex Virus 2
... and clinical isolates we tested (Korom, unpublished). Additional transcripts and alternative splicing of the primary transcript are unlikely mechanisms of C1/C2 generation, because only two spe- cies of mRNA—the ... See full document
13
Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
... cell protein no. 4 (ICP4), a protein known to transactivate viral genes and repress its own ...novo protein synthesis ...viral protein synthesis and the complete repression of this gene by ... See full document
8
Herpes simplex virus 1 protein kinase is encoded by open reading frame US3 which is not essential for virus growth in cell culture.
... Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 606372 Received 16 April 1987/Accepted 11 June 1987 We report that a novel protein kinase previously shown to[r] ... See full document
6
Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ... See full document
12
The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.
... the protein and the RNA were previously reported to be expressed only in cells infected with a deletion mutant or with a mutant carrying a ts lesion in the a 4 gene encoding the infected cell protein ... See full document
9
Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
... Protein extracts from the purified HSV-1 virions were separated on SDS-polyacrylamide gels, and the protein bands were probed in immunoblots with antisera directed against the UL37, ICP8[r] ... See full document
9
The herpes simplex virus Us11 open reading frame encodes a sequence-specific RNA-binding protein.
... Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637 Received 21 February 1990/Accepted 12 April 1990 In this paper we report that a protein encoded by [r] ... See full document
8
Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ... See full document
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