[PDF] Top 20 Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...of simian virus 40 (SV40) DNA has ... See full document
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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... Simian virus 40 (SV40) large tumor (T) antigen is the major regulatory protein that directs the course of viral infection, primarily by interacting with host cell proteins and ... See full document
9
The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...initiate DNA replication, ... See full document
7
Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... Eukaryotic DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD which are defective for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates (i.e., the core origin [0], site I ... See full document
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
6
Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... single-stranded DNA binding activity of T antigen, we generated mutants with single-point substitu- tions within the region identified ...single-stranded DNA binding ... See full document
9
Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... large T and p53 for SV40-mediated cellular transformation is firmly estab- lished (16, 23, 52, 56, 59, 73, 74), although the functional consequences of this interaction are not fully understood and are ... See full document
9
An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... shift DNA-binding experiments showed a reduction in the site II binding activity of T83-708 ...interestingly, binding was still cooperative, as revealed by the titration experiments shown in ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... forked DNA sub- strate is presented in ...the SV40 T-Ag OBD bound to a forked DNA ...forked DNA target used in crystallization indicating duplex DNA and ssDNA ...the DNA ... See full document
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The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication
... Simian virus 40 (SV40) DNA replication is carried out en- tirely by host cell replication proteins, with the exception of one essential viral protein, large tumor (T) ... See full document
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Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.
... 5 0022-538X/88/051598-08$02.00/0 Copyright © 1988, American Society for Microbiology Mutations in the Phosphorylation Sites of Simian Virus 40 SV40 T Antigen Alter Its Origin DNA-Binding[r] ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... The SV40 core origin and structure of SV40 large T-antigen origin binding ...(A) DNA sequence of the SV40 core origin. The 64-base-pair SV40 ... See full document
9
Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ... See full document
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
5
Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain.
... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document
9
Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids.
... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document
8
Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... core origin sequences, in addition to site II, that are required for T-ag assembly ...II-dependent T-ag assembly events, band shift experiments were conducted with a set of oligonucleotides termed ... See full document
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