[PDF] Top 20 Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
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Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
... for quantification of HBV DNA have been brought onto the market and have been found to be useful for the routine diagnostic laboratory (2, 6, 8, ...on PCR, show very limited dynamic ranges (2 to 3 ... See full document
5
Multilaboratory Evaluation of Real Time PCR Tests for Hepatitis B Virus DNA Quantification
... m2000sp automated sample preparation (Abbott Molecular, Des Plaines, IL), TaqMan48 HBV ASR with AmpliPrep automated sample preparation (Roche Diagnostics, Indianapolis, IN), ... See full document
5
Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... HBV DNA in both plasma and serum samples from patients with chronic HBV ...infection. Quantification is linear over the full dynamic range of quanti- fication, which covers values observed in both treated ... See full document
7
Application of real time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia
... a real-time polymerase chain reaction (PCR) method based on SYBR-Green for, measuring HBV DNA in serum and ...general real-time PCR methodology is robust and easy to ... See full document
7
Fast detection of Noroviruses using a real time PCR assay and automated sample preparation
... Different sample volume analyzed could account for the differing ...of PCR-positive ELISA-positive specimens with those of PCR-positive but ELISA-negative ...clinical sample, ...in ... See full document
8
Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... HBV DNA present in samples was calculated using a standard curve generated from HBV plasmid standards that indicated the relation be- tween time-to-positivity (TTP) and input ...HBV DNA ... See full document
10
Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... An advantage with this new method is that the genotype is obtained directly from the PCR without further processing. Moreover, the method was found to have higher precision in genotyping and sensitivity for ... See full document
7
Development of a Real Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C
... tember 2009 to July 2011 were determined as representing DHAV-C infection. Nevertheless, the 16 liver samples collected before September 2009 were negative for DHAV-C and were later identified as representing DHAV-A ... See full document
6
Comparison of Real Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy
... the DNA; beads were transferred by the device through various stringent washes to remove contaminating material before elution into 50 l of the buffer supplied with the ...LC PCR reagents constitute a ... See full document
6
Rapid Quantification of Yersinia enterocolitica in Pork Samples by a Novel Sample Preparation Method, Flotation, Prior to Real Time PCR
... of real-time PCR thermal cycles in the late 1990s has opened up the possibility of accurate quantification of microorganisms in clinical, environmental, and food ...suitable sample ... See full document
6
Development of a TT Virus DNA Quantification System Using Real Time Detection PCR
... the quantification of TTV among HCV- infected patients might be ...TTV DNA levels were similar, irrespective of the severity of liver injury, and were even similar in HCV-infected patients and blood ...TTV ... See full document
5
Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... HBV DNA levels (4, 5, 12, 13, 26, ...HBV DNA level in patients infected with a single genotype was higher than that of patients infected with a mixture of genotypes in the chronic hepatitis B ... See full document
8
Performance Characteristics and Comparison of Abbott and artus Real Time Systems for Hepatitis B Virus DNA Quantification
... in sample volumes: ...obtained DNA concentration. Therefore, DNA prepared from a high sample volume and resuspended in low-elution buffer enhances the detection rate in low-viral-load ...of ... See full document
7
Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real Time PCR Assay
... qualitative PCR was the first method used (8, 18), but this method cannot differentiate between infection and disease in ...quantitative PCR methods have shown that immune-suppressed patients with a high ... See full document
6
Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA
... Epstein-Barr virus (EBV)-associated ...on real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification ...nested PCR samples were used as negative ... See full document
5
Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... clinical time PCR technology based on Taqman ...HBV DNA with wider dynamic range, higher sensitivity and accurate quantification et ...Digital PCR has the potential to highly accurately ... See full document
5
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... of PCR. As an alternative to synthetic fragments of RNA or DNA, housekeeping genes or different pathogens are used as an internal ...the PCR assay than in vitro-transcribed RNA ... See full document
8
COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... the real-time and endpoint PCR systems in the evaluation of viral load changes in HBsAg-positive patients with chronic ...to hepatitis B virus core antigens (HBcAg) (the majority ... See full document
7
Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... HBV DNA levels in international units per milliliter), automated, and rapid, with minimal hands-on time ...HBV DNA, regardless of the HBV genotype or sequence polymor- ...HBV DNA ... See full document
8
Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments
... IC DNA was amplified in a competitive fashion to- gether with potential HBV ...HBV DNA as well as to the increased amounts of primers used in the competitive HBV LC-PCR compared to the amounts used ... See full document
6
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