[PDF] Top 20 A sequence-based approach to identify reference genes for gene expression analysis
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A sequence-based approach to identify reference genes for gene expression analysis
... NEPS reference genes, 12 were represented on the array ...12 genes, probes which had a “ P ” call in 100% of the samples were used for the calculation of the scaling factor with only one ... See full document
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Reference Genes for RT qPCR Analysis of Environmentally and Developmentally Regulated Gene Expression in Alfalfa
... target genes. Normalization of cDNA concentrations with stably expressed genes is needed to minimize the effects of uncontrolled factors such as variation in RNA concentrations or the efficiency of reverse ... See full document
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Partial least squares based gene expression analysis in renal failure
... the gene expression difference between end-stage renal failure patients and healthy ...regression analysis, without considering unaccounted array specific ...PLS based analysis to ... See full document
6
Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage
... of genes have been used in the past as housekeeping genes in cartilage studies in various ...housekeeping gene in studies of human, bovine, porcine, and caprine articular cartilage, including both ... See full document
7
Comparison of normal and pre-eclamptic placental gene expression: a systematic review with meta-analysis
... of gene regulation and expression in the molecular path- ogenesis of the ...placental gene expression is advancing [16 – ...Relative Gene Expression (RGE) in NP and PE placen- ... See full document
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Meta-analysis of microarray data using a pathway-based approach identifies a 37-gene expression signature for systemic lupus erythematosus in human peripheral blood mononuclear cells
... Data sets from five independent microarray studies comparing PBMC samples from SLE patients with those from healthy individuals were obtained from prominent SLE researchers. These data sets are referred to as data sets ... See full document
10
Statistical modelling of transcript profiles of differentially regulated genes
... Our approach to first model and then cluster allows more genes to be considered and potentially a greater insight to understand the ...this approach to a microarray dataset allows the screening of ... See full document
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Identification of stable reference genes for quantitative PCR in koalas
... all reference genes expressed mean pairwise variation value of ...ranked genes. However, in general three reference genes are recommended for accurate normalization in gene ... See full document
8
Selection and validation of reference genes for gene expression analysis in apomictic and sexual Cenchrus ciliaris
... testing reference genes for apomictic gene expression analysis, two plants, Boechera and Brachiaria brizantha, have been used to validate refer- ence genes in their respective ... See full document
9
DAWN: a framework to identify autism genes and subnetworks using gene expression and genetics
... all genes analyzed in periods 3–5 and ...a gene was labeled ‘yes’ if it was identified in any of the four module ...a gene with 0, 1, or at least 2 identified dnLoF mutations, ...rASD genes ... See full document
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Intergenic subset organization within a set of geographically defined viral sequences from the 2009 H1N1 influenza A pandemic
... bioinformatic analysis was performed on the dataset of viral sequences from the 2009 H1N1 influenza pandemic in the state of ...for analysis because 1) its wavelike kinetics has been shown to be associated ... See full document
10
Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures
... candidate reference genes determined by geNorm was compared with Microsoft Excel-based applet termed ...candidate reference gene and ranks the genes according to their ... See full document
8
Selection of reference genes for quantitative real time PCR analysis in chicken embryo fibroblasts infected with avian leukosis virus subgroup J
... gene expression analysis, the selection of stably expressed reference gene (or genes) is an important technical pre- requisite for each individual experimental setting ...the ... See full document
5
Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua
... candidate reference genes for qPCR in ...best reference genes according to the average expression stability (M) or stability values acquired by ΔCt method, geNorm, BestKeeper, Norm- ... See full document
11
Improving knowledge on the activation of bone marrow fibroblasts in MGUS and MM disease through the automatic extraction of genes via a nonnegative matrix factorization approach on gene expression profiles
... 1393 genes were identify through the extraction procedure; we refer to this subset as meta- ...common genes previously ...an analysis on the associated coefficient matrix was per- ...tional ... See full document
16
Evaluation of Reference Genes for Differential Gene Expression Study of Bovine Tuberculosis
... that expression of many housekeeping genes can be influenced by different experimental conditions, which should prevent their use in qPCR assays when those conditions are encountered [6, ...7]. Based ... See full document
8
Differential regulation of two histidine ammonia-lyase genes during Xenopus development implicates distinct functions during thyroid hormone-induced formation of adult stem cells
... If and how HAL2 participates in stem cell formation/ proliferation remains to be investigated. HAL encodes an enzyme known as histidase [28-31]. At biochemical level, histidase catalyzes the first step in the histidine ... See full document
11
Identification of genes and pathways potentially related to PHF20 by gene expression profile analysis of glioblastoma U87 cell line
... net analysis revealed that multiple pathways participate in the occurrence and development of cancer including the p53 signaling pathway, apoptosis, pathways in can- cer, and the TLR signaling ...hybridization ... See full document
12
Isolation and Characterization of a Black PigmentedCorynebacterium sp from a Woman with Spontaneous Abortion
... FIG. 2. Evolutionary distance dendrogram of selected corynebacterial 16S rRNA sequences, including that of Corynebacterium sp. strain CN-1. M. tuberculosis (X55588) and R. dentocariosa (M59055) were chosen as outgroups ... See full document
5
Mining of vaccine-associated IFN-γ gene interaction networks using the Vaccine Ontology
... It is possible to further improve the literature-based network discovery by applying biomedical ontologies. A biomedical ontology represents the consensus-based con- trolled vocabularies of terms and ... See full document
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