[PDF] Top 20 Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
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Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
... This report demonstrates the ability of both recombinant fusion maltose-binding protein MBP-Rep68A produced in Escherichia coli and wild-type wt Rep68 to bind to a linear truncated form [r] ... See full document
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Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.
... D sequence in AAV ITRs is crucial for efficient rescue, selec- tive replication, and successful encapsidation of the AAV ge- nome (39, ...D sequence were removed, the AAV genome could still undergo ... See full document
6
Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome
... deno-associated virus type 2 (AAV2) is a nonpathogenic hu- man parvovirus with a unique biphasic life ...helper virus, AAV2 establishes a latent infection, while in the presence of a ... See full document
9
Characterization of Adenovirus-Induced Inverted Terminal Repeat-Independent Amplification of Integrated Adeno-Associated Virus rep-cap Sequences
... recombinant adeno-associated virus type 2 (rAAV-2) assembly constitute an attractive alternative to transient transfection ...a stable HeLa rep-cap cell clone (HeRC32) and ... See full document
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Mutational Analysis of Adeno-Associated Virus Type 2 Rep68 Protein Endonuclease Activity on Partially Single-Stranded Substrates
... the Rep68 and Rep78 proteins (Rep68/78) of adeno-associated virus type 2 (AAV) cuts at the terminal resolution site (trs) within the hairpin structure formed by the ... See full document
7
Subcellular compartmentalization of adeno-associated virus type 2 assembly.
... tious virus (3, 7, 11, 12, 15, 16, 31, ...Rep78, Rep68, Rep52 and Rep40 accord- ing to their apparent molecular ...and Rep68 play key roles in AAV DNA replication (25, 62) by binding to the ... See full document
12
A novel terminal resolution-like site in the adeno-associated virus type 2 genome.
... The adeno-associated virus 2 (AAV) contains a single-stranded DNA genome of which the terminal 145 nucleotides are palindromic and form T-shaped hairpin ...These inverted ... See full document
7
Efficient Replication of Adeno-Associated Virus Type 2 Vectors: a cis-Acting Element outside of the Terminal Repeats and a Minimal Size
... to sequence positions 4489 to 4695, which is 213 bp upstream of the MVM polyadenylation ...between sequence positions 194 to 1882 ...their binding sites is evident in the AAV2 domain containing the ... See full document
11
Determination of adeno-associated virus Rep68 and Rep78 binding sites by random sequence oligonucleotide selection.
... (AAV), Rep68 and Rep78, have been shown to regulate AAV replication (3–5, 14), transcription (13), and integra- tion (11, 12, ...requires binding of these Rep proteins to tandem repeats of a GAGC ... See full document
5
Rescue and replication of adeno-associated virus type 2 as well as vector DNA sequences from recombinant plasmids containing deletions in the viral inverted terminal repeats: selective encapsidation of viral genomes in progeny virions.
... The adeno-associated virus type 2 (AAV) genome can be successfully rescued from recombinant plasmids following transfection in adenovirus-infected human ...AAV inverted ... See full document
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The Adeno-Associated Virus Type 2 Regulatory Proteins Rep78 and Rep68 Interact with the Transcriptional Coactivator PC4
... The adeno-associated virus type 2 (AAV-2) Rep78/Rep68 regulatory proteins are pleiotropic effectors of viral and cellular DNA replication, of cellular transformation by ... See full document
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Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats.
... RBE sequence contains a set of four imperfect GAGC motifs which we and others (1, 10, 22, 23, 35) had speculated might be the important feature of this element for Rep ...the sequence recog- nized by Rep ... See full document
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Charged-to-Alanine Scanning Mutagenesis of the N-Terminal Half of Adeno-Associated Virus Type 2 Rep78 Protein
... Adeno-associated virus (AAV) type 2 (referred to here as AAV) is a nonpathogenic parvovirus with a linear, single- stranded DNA genome of ...an inverted terminal repeat ... See full document
12
DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... simplex virus (HSV)- encoded replication functions was shown to provide helper activity for productive replication of adeno-associated virus ...AAV type 2 contains a ...145-bp ... See full document
5
Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted repeats
... protein binding site because of steric hindrance. To identify the core sequence, we aligned the Galileo THAP binding site with the P-element THAP sites, together with two further well-de fi ned ... See full document
8
Analysis of the Effects of Charge Cluster Mutations in Adeno-Associated Virus Rep68 Protein In Vitro
... and Rep68 proteins of adeno-associated virus type 2 (AAV) are multifunctional proteins which are required for viral replication, regulation of AAV promoters, and preferential ... See full document
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A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.
... the virus. The creation of plasmid pMBP- Rep68 D has allowed the production of large quantities of rel- atively pure Rep fusion protein for functional analysis ...MBP- Rep68 D has performed ... See full document
7
Analysis of the terminal repeat binding abilities of mutant adeno-associated virus replication proteins.
... To determine whether the mutant Rep proteins produced in plasmidtransfected COS-1 cells were capable of binding to the AAV terminal repeat sequences, gel mobility shift assays were perfo[r] ... See full document
6
Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants.
... Among these mutants, we distinguished at least three phenotypes: i mutants that were defective in their ability to rescue or replicate the AAV genome from the recombinant plasmids rep mu[r] ... See full document
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Nucleotide sequence and organization of the adeno-associated virus 2 genome.
... The sequence AAUAAA forms part of the recognition site for polyade- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The large open reading frames on the left half of th[r] ... See full document
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