[PDF] Top 20 Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... of DNA is displaced from the channel is not clear, but it probably involves a conformation change of the T-anti- gen ...nucleotide binding domain, can also form both rings and filaments (81), ... See full document
9
Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... comparing T-antigen binding to the DNA fork and DNA bubble, reaction mixtures containing ...the DNA fork or bubble substrates, 700 ng of T antigen, ...duplex ... See full document
8
Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...large ... See full document
7
Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... Since binding of T antigen to the p68 subunit was not de- tected in the far-Western assay shown in ...mutant T antigens immobilized on an immunoaffinity resin were incu- bated with purified ... See full document
10
The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
6
Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity.
... Second, wild-type T antigen's nonspecific DNA binding, but not its origin binding, was significantly inhibited with a number of monoclonal antibodies PAb1O4, PAb114, PAb252, PAb4O2, PAb4[r] ... See full document
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The T-antigen-binding domain of the simian virus 40 core origin of replication.
... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document
7
Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document
8
Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.
... 5 0022-538X/88/051598-08$02.00/0 Copyright © 1988, American Society for Microbiology Mutations in the Phosphorylation Sites of Simian Virus 40 SV40 T Antigen Alter Its Origin DNA-Binding[r] ... See full document
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DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state.
... i They clearly show the relationship between different subclasses, i.e., the DNAbinding, monomeric nuclear high-salt fractions on one hand and the nonbinding, oligomeric fractions on the[r] ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) shows ... See full document
11
Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.
... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ... See full document
8
Removal of serine phosphates from simian virus 40 large T antigen increases its ability to stimulate DNA replication in vitro but has no effect on ATPase and DNA binding.
... These authors found no effect of dephosphorylation of large T antigen with alkaline phosphatase on ATPase activity and only a "slight" stimulatory effect on DNA binding when using origin[r] ... See full document
8
Simian Virus 40 DNA Replication Is Dependent on an Interaction between Topoisomerase I and the C-Terminal End of T Antigen
... origin-containing DNA. The separated labeled “top” strand of this DNA was detected and quantitated by gel electrophoresis ...origin DNA like WT T antigen ...normal activity, ... See full document
10
Identification of simian virus 40 T-antigen residues important for specific and nonspecific binding to DNA and for helicase activity.
... 4858 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified three regions called elements in the DNA-binding domain of simian virus 40 large tumo[r] ... See full document
8
Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... ssDNA binding activity of mutant T ...resulting single strands in their appropriate sites in and around the helicase ...ssDNA binding assay using a 55-mer labeled oligonucleotide in the ... See full document
10
Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the T-Ag OBD, we screened for crystal formation be- tween the T-Ag OBD with a range of DNA templates contain- ing regions of ...the T-Ag OBD bound to the single-stranded region ... See full document
10
Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document
8
Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
... We previously reported 28 that only a very small fraction of T Ag binds with a higher salt-sensitive affinity to SV40 DNA than to calf thymus DNA-cellulose under these conditions and tha[r] ... See full document
5
Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD spiral hexamer is con- figured such that many of the A1 and B2 residues that interact preferentially with ssDNA in the NMR studies ...disrupt T-ag’s helicase (green) and unwinding (orange) ... See full document
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