Conditions used for Reverse-Phase HPLC Analysis
Study of method validation on ramipril with reverse phase HPLC method
... precise reverse phase high performance liquid chromatographic method has been developed and subsequently validated for the estimation of Ramipril in bulk and its pharmaceutical dosage ...mobile phase ...
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Simultaneous estimation of lornoxicam and paracetamolin tablets by reverse phase HPLC method
... . fig. 2: Calibration curve forParacetamol Preparation of standard stock solutions and standard calibration curves: Standard stock solution of LOR and PAR were prepared separately by transferring 10 mg of LOR and 10 mg ...
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METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF TINIDAZOLE BY REVERSE PHASE HPLC TECHNIQUE
... It has a plasma elimination half life of 12.3 hours and about 63% of this drug is eliminated through hepatic metabolism 9, 10 . Its dosage is independent of sex, race and hepatic metabolism however it is not recommended ...
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CHARACTERISATION OF BIOACTIVE COMPOUNDS IN COSTUS SPECIOSUS (KOEN) BY REVERSE PHASE HPLC
... This second extraction ensures complete removal of alcohol soluble substances. Both the extracts were pooled, filtered through Whatman No. 41 filter paper and the volume was raised with 80% ethanol or reduced by rotary ...
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Identification of Antihyperlipidemic components in Aloe vera through reverse phase HPLC
... Aloe vera (L) has a long history of both as an ornamental plant and for herbal medicine being used as a valuable ingredient for the food, pharmaceutical and cosmetic industries (Eshun and He, 2004; Surjushe et ...
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METHOD DEVELOPMENT AND VALIDATION OF RELATED SUBSTANCES IN ASENAPINE TABLETS BY REVERSE PHASE HPLC
... Mobile phase composition, Extraction and Flow Variation The test preparation was injected under normal condition ...altered conditions column temperature 40±5°C, flow rate ...mobile phase composition ...
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VALIDATION OF A STABILITY INDICATING REVERSE PHASE HPLC METHOD FOR DETERMINATION OF CANAGLIFLOZIN API
... ABSTRACT A new simple, rapid and sensitive RP-HPLC method has been developed for the determination of Canagliflozin Active Pharmaceutical Ingridient (API). The method employs X-bridze C18 column (150mm×4.6 mm, 5mm ...
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DEVELOPMENT AND VALIDATION OF REVERSE-PHASE HPLC METHOD FOR ESTIMATION OF KETOCONAZOLE IN BULK DRUG
... Assay of tablets of Ketoconazole were perfomed. Twenty tablets of each company of strength 200 mg were weighed and ground to a fine powder. A quantity of tablet powder equivalent to 10 mg of Ketoconazole was transferred ...
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Validated reverse phase HPLC method for the determination of topotecan in pharmaceutical dosage forms
... Few HPLC methods for quantitative determination of topotecan in formulations were reported in the literature and these reports mainly included the determination of topotecan and its metabolites in biological ...
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A simple and validated reverse phase HPLC method for the determination of Blonanserin in pharmaceutical dosage forms
... 276 suitability parameters were also calculated. To demonstrate specificity in the presence of excipients used in formulation, blonanserin was spiked in drug product; chromatogram was observed and compared with ...
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DEVELOPMENT AND VALIDATION OF REVERSE PHASE HPLC METHOD FOR ESTIMATION OF HAMYCIN AND KETOCONAZOLE IN PHARMACEUTICAL CREAM
... Hamycin bind to ergosterol, the major membrane lipid in fungal cells. 7, 8 FIG. 2: STRUCTURE OF HAMYCIN MATERIALS AND METHODS: All the reagents used were of HPLC grade and analytical grade from Rankem, ...
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VALIDATION OF ABACAVIR SULFATE IN PHARMACEUTICAL DOSAGE BY REVERSE PHASE HPLC WITH INTERNAL STANDARD METHOD
... isocratic HPLC method was developed and validated for the assay of abacavir sulfate in pharmaceutical dosage ...mobile phase composition consists of (38:62 v/v) of methanol and 10ml of potassium dihydrogen ...
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REVERSE PHASE HPLC METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF MELOXICAM IN BULK AND PHARMACEUTICAL FORMULATION
... the analysis of MEL in pharmaceuticals like, UV spectrophotometry 4-5 , fluorimetry 4 , capillary electrophoresis 6 , pulse polarography 7 , electrochemical oxidation 8 , electrochemical reduction 9 and voltametry ...
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VALIDATION OF REVERSE PHASE-HPLC METHOD FOR THE QUANTITATIVE ESTIMATION OF NORFLOXACIN IN PURE AND PHARMACEUTICAL FORMULATION
... excipients used in the preparations. The proposed chromatographic method can be used to analyse a large number of samples each day in analytical laboratories, since they need same reversed-phase ...
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Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk and Pharmaceutical Dosage Forms
... Ten tablets each containing 50 mg were weighed accurately and powdered. A quantity equivalent to 50 mg of Naftopidil was weighed accurately and transferred to 50 ml volumetric flask containing 30 ml of methanol. The ...
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Validated Reverse Phase HPLC Method for The Determination of Pemetrexed Disodium in Pharmaceutical Dosage Forms
... Method validation System suitability The system suitability was assessed by replicate analysis of six injections of the drug at a concentration of 0.251 mg/ml. The acceptance criteria were not more than 2% for the ...
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Development of a New Rapid, Efficient and Reproducible Reverse Phase - HPLC Method for the Analysis of Decitabine in Bulk and Tablet Dosage Form
... reproducible reverse phase-HPLC method for the analysis of Decitabine in bulk drug and tablet dosage ...mobile phase containing ACN : Acetate buffer (55:45), adjusted to pH ...further ...
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A new reverse phase HPLC method for quick quantificationof all four isomers of natural vitamin-E oil in a single analysis
... by reverse phase High Performance Liquid Chromatography within short ...were used. And even mobile phase has used only Acetonitrile and ...
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Simultaneous Estimation of Ascorbic Acid and Calcium Pantothenate in Multivitamin and Multimineral Tablets by Reverse-Phase HPLC
... chromatographic conditions for the estimation of vitamins in literature, it was found that the mobile phases in existing methods were very much complicated with gradient elution system, ion pairing reagents were ...
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Development and validation of a Reverse Phase HPLC method for the determination of Rosiglitazone Maleate in tablet dosage form
... each analysis by comparing the blank chromatogram with the chromatogram obtained for the drug spiked with internal standard(placebo) to trace out the interfering ...
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