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Leukocyte telomere length variation due to DNA extraction method

Leukocyte telomere length variation due to DNA extraction method

... different extraction methods could yield misleading ...the use of different extraction methods across multiple time- points should be ...as DNA purity could influence telomere length, it could ...

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The MAGi DNA extraction method for fresh tissues and dry seeds

The MAGi DNA extraction method for fresh tissues and dry seeds

... MAGi DNA extraction method completes in approximately 1 h and could be used in small scale (mini) using 100 mg fresh issue or in midi scale using 1 g ...This method is also suitable for ...

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Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)

Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)

... Present-day DNA-based molecular studies are use- ful tools with a wide-range of applications in different biological ...of DNA [3, 4] to successfully solve taxonomic and phy- logenetic controversies ...

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A New and Efficient Method for DNA Extraction from Human Skeletal Remains Usable in DNA Typing

A New and Efficient Method for DNA Extraction from Human Skeletal Remains Usable in DNA Typing

... bone extraction proto- cols used in the forensic laboratories involve an incubation period of bone powder in extraction buffer for proteinase digestion, followed by the collection of the su- pernatant, and ...

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Fast and Efficient DNA Extraction Method for a Plumeria acuminata with Medicinal Potential

Fast and Efficient DNA Extraction Method for a Plumeria acuminata with Medicinal Potential

... obtaining DNA samples of ...the DNA preparations. The polysaccharide is hindered by use of high amount of ...interfere DNA isolation ...to DNA separation in the supernatant and ...

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An Efficient DNA Extraction Method for Lactobacillus casei, a Difficult-to-Lyse Bacterium

An Efficient DNA Extraction Method for Lactobacillus casei, a Difficult-to-Lyse Bacterium

... PCR reaction was performed using 5 μL of the extract- ed DNA with 25 μL of ready-to-use PCR master mix 2x (PR901638, SinaClon, Iran), 2.5 μL (20 pmol/μL) of each primer and dH 2 O till 50 μL volume was ...

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Report on the Validation of a DNA Extraction. Method for Dried-killed Bacterial Biomass

Report on the Validation of a DNA Extraction. Method for Dried-killed Bacterial Biomass

... of DNA The size of the extracted DNA was evaluated by agarose gel electrophoresis; 5 µL of the DNA solution were analysed on a ...genomic DNA samples extracted as described above appeared as ...

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Unveiling Cost Burden Interdependencies with the Use of the Hypothetical Extraction Method

Unveiling Cost Burden Interdependencies with the Use of the Hypothetical Extraction Method

... sequential extraction of the complete cost structures for each and every sector of the economy; in this specific case H = i for i = 1, 2,  , 35 which means 35 ...the selected group of industries, while the ...

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DNA extraction from olive oil and its use in the identification of the production cultivar

DNA extraction from olive oil and its use in the identification of the production cultivar

... Abstract DNA recovery from food samples might be of great importance when the raw material used in the production process has to be ...reliable DNA extraction method for extra virgin olive oil ...

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Validation of a DNA extraction method using diluted ATL as an extraction buffer with the Qiagen® Lyse&Spin Basket Kit

Validation of a DNA extraction method using diluted ATL as an extraction buffer with the Qiagen® Lyse&Spin Basket Kit

... more DNA in the substrate than the currently used ...of DNA extracted when the starting amount of DNA is ...of DNA, QC stains and 1:2 saliva dilutions, showed a significant difference between ...

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Method specific calibration corrects for DNA extraction method effects on relative telomere length measurements by quantitative PCR

Method specific calibration corrects for DNA extraction method effects on relative telomere length measurements by quantitative PCR

... to use a completely consistent approach and extract DNA using the same method within a ...the use of long-term longitudinal archived samples, in which samples may have been stored or ...

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Determining the Promoter Region in the DNA using ke-Rem (ke-Rule Extraction Method)

Determining the Promoter Region in the DNA using ke-Rem (ke-Rule Extraction Method)

... (ke-Rule Extraction Method) was upgraded to have the ability to obtain IF-THEN rules from a given set of ...to further provide rules that are more commonly ...

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Extraction of human genomic DNA from whole blood using a magnetic microsphere method

Extraction of human genomic DNA from whole blood using a magnetic microsphere method

... magnetic extraction is a simple, automatic, and highly efficient method for separating biological mole­ cules, performing immunoassays, and other ...genomic DNA. Extracting genomic DNA by ...

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Comparative Analysis of Traditional versus Authentic Teaching Method in DNA Extraction to Biology Students

Comparative Analysis of Traditional versus Authentic Teaching Method in DNA Extraction to Biology Students

... uses DNA extraction as the ...traditional method in teaching by lecturing and the second treatment involved using the authentic method by showing the actual process of DNA ...authentic ...

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An Efficient Method for Extraction of Genomic DNA from Insect Gut Bacteria - Culture dependent

An Efficient Method for Extraction of Genomic DNA from Insect Gut Bacteria - Culture dependent

... extracted DNA In order to check the efficiency and reliability of the genomic DNA isolated by our proposed method, DNA was subjected to restriction digestion and RAPD-PCR ...genomic DNA ...

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Simple and quick method of DNA extraction from different parts of plant for PCR amplification

Simple and quick method of DNA extraction from different parts of plant for PCR amplification

... the DNA molecular marker based genetic ...genomic DNA is the prerequisite for molecular biology research. An efficient DNA extraction method should be simple, rapid and affordable to ...

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High Quality DNA Obtained from a Single Seed of Vitis vinifera L  Using Rapid DNA  Extraction Method

High Quality DNA Obtained from a Single Seed of Vitis vinifera L Using Rapid DNA Extraction Method

... 3.1. DNA Extraction of Single Seed of Grapevine Approximately 20 - 35 mg of dry weight of single grapevine seed was used for the genomic DNA isolation us- ing two DNA extraction ...

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A Modified Extraction Method of Circulating Free DNA for Epidermal Growth Factor Receptor Mutation Analysis

A Modified Extraction Method of Circulating Free DNA for Epidermal Growth Factor Receptor Mutation Analysis

... MPC method was more intact than by Qiagen kit ...different extraction method of MPC or Qiagen kit on EGFR mutation ...MPC method is superior to Qiagen kit on plasma cfD- NA extraction ...

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Technical Note: Improved Method for Rapid DNA Extraction of Mastitis Pathogens Directly from Milk

Technical Note: Improved Method for Rapid DNA Extraction of Mastitis Pathogens Directly from Milk

... high-quality DNA from the target bacteria found in milk, however, is often problematic and may require overnight selective-enrichment procedures (Phuektes et ...pathogenic DNA present in a typical sample. ...

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Extraction of DNA  Extraction of DNA

Extraction of DNA Extraction of DNA

... of DNA • The DNA can be stained by the inclusion of ethidium bromide in the gel, or by soaking the gel in a solution of ethidium bromide after ...The DNA shows up as an orange band on illumination by ...

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