Multilocus sequence typing (MLST)
Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex
... single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least ...
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Comparison of Single and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin Resistant Staphylococcus aureus
... Different typing methods provide different information. Multilocus sequence typing (MLST) reveals slowly accumulating changes in conserved genes that reflect long- term evolutionary ...
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Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... portable typing methods, the sequencing of the p37 gene and a multilocus sequence typing (MLST) ...The MLST scheme was developed with the sequenced genomes of the ...of ...
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Multilocus sequence typing (MLST), porA and flaA typing of Campylobacter jejuni isolated from cats attending a veterinary clinic
... selected multilocus sequence typing (MLST) ...using MLST, isolates are char- acterized by partial sequencing of seven housekeep- ing genes whose allele combination groups strains into ...
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Major Uropathogenic Escherichia coli Strain Isolated in the Northwest of England Identified by Multilocus Sequence Typing
... Multilocus sequence typing (MLST) is a DNA sequence- based method that has been used to study the population biology of pathogenic microorganisms and provides an under- standing of the ...
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Development and Application of a Multilocus Sequence Typing Scheme for Streptococcus gallolyticus subsp gallolyticus
... a multilocus sequence typing (MLST) ...50 sequence types. Our first results highlight the importance of this MLST scheme for investigating the epidemiology, transmission ...
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Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... developed multilocus sequence typing (MLST) approach with a well-established molecular typing technique, pulsed-field gel electrophoresis (PFGE), for subspecies differen- tiation of ...
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Multilocus Sequence Typing of Candida glabrata Reveals Geographically Enriched Clades
... 42), multilocus enzyme electrophoresis (MLEE) (5), and fingerprinting with complex DNA probes ...direct sequence comparison for typing ...direct sequence comparison, we developed a ...
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Multilocus Sequence Typing System for Group B Streptococcus
... A multilocus sequence typing (MLST) system was developed for group B streptococcus ...or sequence type (ST) for each ...GBS MLST was set up and can be accessed at ...GBS ...
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Multilocus Sequence Typing Analysis of Human and Animal Clostridium difficile Isolates of Various Toxigenic Types
... Multilocus sequence typing (MLST) has been recently de- veloped for the study of clonal relationships within bacterial populations and has been successfully used for population ge- netics and ...
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The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus
... bacterial typing; however, few account for whole genome diversity or provide the opportunity for future application of new computational ...techniques. Multilocus sequence typing (MLST) ...
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Estimating Recombinational Parameters in Streptococcus pneumoniae From Multilocus Sequence Typing Data
... Multilocus sequence typing (MLST) is a highly discriminatory molecular typing method that defines isolates of bacterial pathogens using the sequences of ⵑ450-bp internal fragments of ...
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Semiautomation of Multilocus Sequence Typing for the Characterization of Clinical Isolates of Neisseria meningitidis
... by typing and ...using multilocus sequence typing (MLST) as a routine method for the characterization of isolates of Neisseria ...meningitidis. MLST produces nucleotide ...
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Multilocus Sequence Typing Scheme for Enterococcus faecalis Reveals Hospital Adapted Genetic Complexes in a Background of High Rates of Recombination
... A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus ...faecalis. MLST of 110 ...
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Comparison of Microsatellite Length Polymorphism and Multilocus Sequence Typing for DNA Based Typing of Candida albicans
... each deoxynucleotide triphosphate, 10 pmol of each primer, and 1.25 U of FastStart Taq DNA polymerase (Roche Applied Science). The PCR conditions consisted of an initial denaturation step of 6 min at 95°C, followed by 35 ...
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Characterization of Epidemiologically Unrelated Acinetobacter baumannii Isolates from Four Continents by Use of Multilocus Sequence Typing, Pulsed Field Gel Electrophoresis, and Sequence Based Typing of blaOXA 51 like Genes
... of epidemiologically unrelated A. baumannii isolates collected from 22 countries over four continents, Europe, Asia, Africa, and South America (8). In addition, because bla OXA-51-like genes are endogenous to A. ...
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Streptococcus mutans Clonal Variation Revealed by Multilocus Sequence Typing
... with sequence homologies that are specific to ...recent MLST schemes, because it is important that the selection of the genes to be sequenced in an MLST scheme developed for a partic- ular bacterial ...
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A Trilocus Sequence Typing Scheme for Hospital Epidemiology and Subspecies Differentiation of an Important Nosocomial Pathogen, Enterococcus faecalis
... and multilocus enzyme electro- phoresis ...repetitive sequence-based PCR (25, 35), multilocus variable-number tandem-repeat analysis (49, 54), pulsed-field gel electrophoresis (PFGE) (10, 12, 49), ...
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Genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from conventional broiler flocks and the impacts of sampling strategy and laboratory method
... MLST. MLST was performed as described previously ...nucleotide sequence of the amplicons was determined using published oligonucleotide primers and reaction conditions (30, ...Nucleotide ...
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Clonal Characterization of Staphylococcus aureus by Multilocus Restriction Fragment Typing, a Rapid Screening Approach for Molecular Epidemiology
... of multilocus sequence typing (MLST) in which sequence variations in the MLST housekeeping gene loci are detected by restriction fragment pattern analysis rather than sequencing; ...
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