PBS, primer binding site
Genetic Analysis of a Unique Human Immunodeficiency Virus Type 1 (HIV-1) with a Primer Binding Site Complementary to tRNAMet Supports a Role for U5-PBS Stem-Loop RNA Structures in Initiation of HIV-1 Reverse Transcription
... FIG. 1. RNA modeling of wild-type and mutant viral U5-PBS and U5-PBS/ tRNA. (A) The viral RNA sequence of nucleotides 1 to 230 encompassing the R-U5-PBS from the wild-type (HXB2) and mutant ...
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Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer.
... that PBS mutations designed not to match any naturally occurring tRNA molecule result in a strong impairment of vector transduction ...altered primer, we have used single cycle replication of murine ...
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A preferred region for recombinational patch repair in the 5' untranslated region of primer binding site-impaired murine leukemia virus vectors.
... tRNA-Gln1,2-specific primer and a U5 primer matching Akv positions 69 to 96 ...the PBS-Gln-harboring proviruses ...harboring PBS-Gln1,2 and parent ...the PBS (positions 145 to 162), and ...
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Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses
... introduced PBS mutations were genetically stable during in vivo replication and tumor induction, purified genomic DNAs from all tumors were subjected to PCR am- plification with primers specific for the ecotropic ...
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Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
... of PBS-containing proviral DNA. Analysis of the proviral PBS sequences from infected SupT1 cells was carried out as previously described ...the PBS was amplified by PCR from approximately 10 mg of ...
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Proteolytic Activity, the Carboxy Terminus of Gag, and the Primer Binding Site Are Not Required for Pol Incorporation into Foamy Virus Particles
... The activities of the HFV Pol domains have been studied in vitro. The RT activity from a foamy virus was first demon- strated in 1971 (29), and later template-primer sets for endog- enous RT activity were ...
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Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication.
... During early stages of infection, the two PBS mutants, in conjunction with tRNA1Ls and tRNAPhe, appar- An early, critical step in the human immunodeficiency virus type HIV-1 life cycle i[r] ...
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Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers.
... tRNA primer is base paired to the primer binding site (PBS), a complementary region near the 59 end of the viral ...the PBS is not the basis for packaging of the specific ...
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Leader Sequences Downstream of the Primer Binding Site Are Important for Efficient Replication of Simian Immunodeficiency Virus
... the PBS at position 303, the DIS palindrome at position 419, and the Gag start codon at position ...donor site at position 466 is marked by a solid ...
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HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
... cleavage site of Cas9 ...the PBS were not ...wild-type PBS, leaving a repeat of a partial PBS sequence immediately downstream, and thus disrupt the binding of sgPBS3 to its target DNA ...
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Role of Stem B, Loop B, and Nucleotides next to the Primer Binding Site and the Kissing-Loop Domain in Human Immunodeficiency Virus Type 1 Replication and Genomic-RNA Dimerization
... The primer binding site (PBS) and the gag gene are separated by 136 nt (the L sequence) which fold into stem B, loop B, the klh, and conceivably five other regions, such as nt 200 to 226, stem ...
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Identification of sequences downstream of the primer binding site that are important for efficient replication of human immunodeficiency virus type 1.
... the PBS were involved in reverse transcription in cell-free systems (24, 25, 33), we asked whether the modifica- tions introduced into our constructs would result in impaired generation of viral ...with ...
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Changes in Rous Sarcoma Virus RNA Secondary Structure near the Primer Binding Site upon tRNATrpPrimer Annealing
... facilitate primer annealing of their respective primers to the corresponding viral RNA both in vitro and in vivo (6, 9, 21, ...for primer annealing in these ...the PBS) are seen in both the U5-Leader ...
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Lack of Shielding of Primer Binding Site Silencer-Mediated Repression of an Internal Promoter in a Retrovirus Vector by the Putative Insulators scs, BEAD-1, and HS4
... the primer binding site (PBS) region of the 5⬘ untranslated region (UTR), which binds a proline (Pro) tRNA primer for initiation of reverse ...the PBS (the B2 PBS) was ...
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The roles of the human immunodeficiency virus type 1 Pol protein and the primer binding site in the placement of primer tRNA(3Lys) onto viral genomic RNA.
... DNA sequencing of the PBS and its flanking region after infection of MT-2 cells by mutated viruses. MT-2 cells infected by mutated viruses (BH10-PBS2, BH10- PBS4, and BH10-PBS8), which had been treated with ...
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Placement of tRNA primer on the primer-binding site requires pol gene expression in avian but not murine retroviruses.
... pol 2 mutant of MuLV. The results presented above indicate that pol gene expression is required for efficient placement of tRNA on the PBS in ALV. To determine whether this is generally true for retroviruses, we ...
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Stem cell factor binding to retrovirus primer binding site silencers.
... KpnI site at proviral nt 480 (viral nt 32); 5 9 noncoding sequences corresponding to M-MuLV viral nt 32 to 212 from an endogenous murine retrovirus (7), which alters the M-MuLV PBS from one for proline ...
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The Kissing-Loop Motif Is a Preferred Site of 5′ Leader Recombination during Replication of SL3-3 Murine Leukemia Viruses in Mice
... functional primer binding site (PBS) sequences (13, ...the PBS, normal vector transduction is impaired, thereby facilitating the selec- tion of rare transduction events, some of which ...
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Primer Binding Site-Dependent Restriction of Murine Leukemia Virus Requires HP1 Binding by TRIM28
... The results presented here strengthen the argument that TRIM28 activity is required for PBS-mediated silencing in ES FIG. 3. (A) Repression of B2 versus that of PRO MLV. RAT2, F9, TRIM28 ⫹/⫺ , and TRIM28 HP1box/⫺ ...
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Identification of Critical Elements in the tRNA Acceptor Stem and TΨC Loop Necessary for Human Immunodeficiency Virus Type 1 Infectivity
... the primer binding site (PBS) to initiate reverse transcription ...tRNA primer (4, 9, 10, 16). Substitution of the HIV type 1 (HIV-1) PBS with sequence complementary to ...
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