Primer binding site (PBS)
Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers.
... tRNA primer is base paired to the primer binding site (PBS), a complementary region near the 59 end of the viral ...the PBS is not the basis for packaging of the specific ...
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Leader Sequences Downstream of the Primer Binding Site Are Important for Efficient Replication of Simian Immunodeficiency Virus
... the primer binding site (PBS) of ...the PBS and the dimerization initiation site (DIS), while the other two were located within two distinct Gag proteins, ...by ...
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Placement of tRNA primer on the primer-binding site requires pol gene expression in avian but not murine retroviruses.
... The primer for this synthetic event is a molecule of cellular tRNA, which is annealed by its 3 * 18 nucleotides to a region of the genomic RNA termed the primer-binding site (PBS); the ...
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Lack of Shielding of Primer Binding Site Silencer-Mediated Repression of an Internal Promoter in a Retrovirus Vector by the Putative Insulators scs, BEAD-1, and HS4
... the primer binding site (PBS) region of the 5⬘ untranslated region (UTR), which binds a proline (Pro) tRNA primer for initiation of reverse ...the PBS (the B2 PBS) was ...
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Stem cell factor binding to retrovirus primer binding site silencers.
... and binding conditions, we have examined the binding of an embryonal carcinoma (EC) cell factor, binding factor A, to a stem cell-specific silencer which acts at the DNA level and overlaps the ...
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Role of Stem B, Loop B, and Nucleotides next to the Primer Binding Site and the Kissing-Loop Domain in Human Immunodeficiency Virus Type 1 Replication and Genomic-RNA Dimerization
... The primer binding site (PBS) and the gag gene are separated by 136 nt (the L sequence) which fold into stem B, loop B, the klh, and conceivably five other regions, such as nt 200 to 226, stem ...
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Identification of sequences downstream of the primer binding site that are important for efficient replication of human immunodeficiency virus type 1.
... (nt) primer binding site (PBS), located within the 5 * region of viral genomic RNA, to which the host cell-derived tRNA primer is annealed and also involves viral genomic sequences ...
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Primer Binding Site-Dependent Restriction of Murine Leukemia Virus Requires HP1 Binding by TRIM28
... factor binding to the viral enhancers (9, 12) and in larger part due to repressive trans-acting factors in these cell types (1, 4, 19, ...The primer binding site (PBS) of MLV is a major ...
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Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
... the primer binding site (PBS) as a primer (2, ...The PBS is an 18-nucleotide sequence located near the 59 end of the viral RNA genome that is complementary to the 3 9 -termi- nal ...
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Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer.
... a primer for the viral reverse transcrip- tase ...the primer-binding site (PBS), a complementary 18-bp sequence near the 5 9 end of the viral RNA ...tRNA primer molecule is ...
5
Changes in Rous Sarcoma Virus RNA Secondary Structure near the Primer Binding Site upon tRNATrpPrimer Annealing
... the primer binding site in the 5 ⴕ untranslated region of Rous sarcoma virus (RSV) RNA play an integral role in multiple viral replications steps including reverse transcription, DNA integration, and ...
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A preferred region for recombinational patch repair in the 5' untranslated region of primer binding site-impaired murine leukemia virus vectors.
... of primer binding site-impaired Akv murine leukemia virus-based retroviral vectors from the murine packaging cell lines C -2 and V E was ...a primer binding site sequence ...
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Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication.
... During early stages of infection, the two PBS mutants, in conjunction with tRNA1Ls and tRNAPhe, appar- An early, critical step in the human immunodeficiency virus type HIV-1 life cycle i[r] ...
9
HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
... cleavage site of Cas9 ...the PBS were not ...wild-type PBS, leaving a repeat of a partial PBS sequence immediately downstream, and thus disrupt the binding of sgPBS3 to its target DNA ...
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The roles of the human immunodeficiency virus type 1 Pol protein and the primer binding site in the placement of primer tRNA(3Lys) onto viral genomic RNA.
... DNA sequencing of the PBS and its flanking region after infection of MT-2 cells by mutated viruses. MT-2 cells infected by mutated viruses (BH10-PBS2, BH10- PBS4, and BH10-PBS8), which had been treated with ...
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Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses
... Gln PBS (Table 2). Whereas the sequences flanking the Lys3 PBS were identical to those in the original SL3-3-Lys3 mutant, all the sequences harboring a Gln PBS also contained the endogenous proviral ...
6
Proteolytic Activity, the Carboxy Terminus of Gag, and the Primer Binding Site Are Not Required for Pol Incorporation into Foamy Virus Particles
... The activities of the HFV Pol domains have been studied in vitro. The RT activity from a foamy virus was first demon- strated in 1971 (29), and later template-primer sets for endog- enous RT activity were ...
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Genetic Analysis of a Unique Human Immunodeficiency Virus Type 1 (HIV-1) with a Primer Binding Site Complementary to tRNAMet Supports a Role for U5-PBS Stem-Loop RNA Structures in Initiation of HIV-1 Reverse Transcription
... oratory have shown that even in the wild-type genome, muta- tion in this region resulted in a virus which reverts to restore the CCCT motif (31). What is the importance of this interac- tion? One explanation could be ...
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Identification of Critical Elements in the tRNA Acceptor Stem and TΨC Loop Necessary for Human Immunodeficiency Virus Type 1 Infectivity
... the primer binding site (PBS) to initiate reverse transcription ...tRNA primer (4, 9, 10, 16). Substitution of the HIV type 1 (HIV-1) PBS with sequence complementary to ...
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Minimal sequence requirements of a functional human immunodeficiency virus type 1 primer binding site.
... Transfection of the wild-type proviral genome, pHXB2wild type, into COS-1 cells and subsequent coculture with SupTl cells resulted in the production of infectious virus as measured by p2[r] ...
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