[PDF] Top 20 Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
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Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... a major question regarding the herpesvirus replisome: how does one assemble two DNA polymerases for coordinated leading and lagging strand synthesis? The UL8 subunit of UL5- UL8-UL52 reportedly binds to ... See full document
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... Cloning the ICP8 mutants. The goal of this study was to determine the region of ICP8 responsible for the cooperativity exhibited by the molecule in ssDNA binding. ICP8 has a marked tendency to aggregate upon ... See full document
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Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus.
... In addition to cell lines which complemented ts mutants, two lines, U-27 and U-35, significantly inhibited plaque formation by wild-type virus, contained 30 and 100 copies of the ICP8 ge[r] ... See full document
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N-ethylmaleimide inhibition of the DNA-binding activity of the herpes simplex virus type 1 major DNA-binding protein.
... To determine whether this was indeed the case, equilibrium dialysis experiments with ICP8 and [32P]dT1o were done in the presence and absence of NEM as described DISCUSSION The results p[r] ... See full document
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Origin Binding Protein-Containing Protein-DNA Complex Formation at Herpes Simplex Virus Type 1 oriS: Role in oriS-Dependent DNA Replication
... additional protein components of complexes B and C are therefore likely to be cellular in ...cellular protein known to affect the efficiency of HSV origin function (10), may be a component of complex ...HSV ... See full document
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... (Fig. 1) could be used as a reporter to identify and map domains of ICP8 that are involved in, or are conformationally altered by, protein-protein and/or pro- tein-DNA ...coli DNA poly- ... See full document
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Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
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Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.
... 727 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The interaction of the herpes simplex virus type 1 HSV-1 major DNA-binding protein, infected-cell polypeptide 8 ICP8[r] ... See full document
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In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
... 31 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex vi[r] ... See full document
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Relationship between TATA-binding protein and herpes simplex virus type 1 ICP4 DNA-binding sites in complex formation and repression of transcription.
... to 1 27 rela- tive to the transcription start site ...of DNA inserted between the TATA box and the ICP4-binding site ...linker DNA increased the distance between the TATA box and the ... See full document
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DNA binding by the herpes simplex virus type 1 ICP4 protein is necessary for efficient down regulation of the ICP0 promoter.
... Regulation of the herpes simplex virus type 1 late Y2 glycoprotein c gene: sequences between base pairs 34 to +29 control transient expression and responsiveness to transactivation by th[r] ... See full document
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Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... origin binding activity of other viral replication initiator proteins, we sought to determine whether the phosphorylation state of OBP affects its ability to form protein-DNA complexes at its ... See full document
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Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... and DNA sequencing. pFastBac BamHI/EcoRI fragments encoding wild-type and mutant UL9 genes were subcloned into the pCDNA1 vector (Invitrogen) under a cytomegalovirus promoter for in vivo studies in ... See full document
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The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.
... 2428 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The possible identity of the herpes simplex virus type 1 HSV-1 65K 65,000-Mr virion protein which stimulates transc[r] ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... the protein was purified to near homogeneity using a glutathione-Sepharose ...57-kDa protein to a new, 48-kDa ...The protein was crystallized at 25°C using the vapor diffusion method, with the drop ... See full document
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Purification of the herpes simplex virus type 1 65-kilodalton DNA-binding protein: properties of the protein and evidence of its association with the virus-encoded DNA polymerase.
... 8 Purification of the Herpes Simplex Virus Type 1 65-Kilodalton DNA-Binding Protein: Properties of the Protein and Evidence of Its Association with the Virus-Encoded DNA Polymerase MICHA[r] ... See full document
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DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding
... N-terminal domain of UL9 is depicted as an open box, and the C terminus is depicted as a gray ...the protein synthesized from each truncation is indicated at the right side of the ...Prestained ... See full document
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Characterization of major recognition sequences for a herpes simplex virus type 1 origin-binding protein.
... 4096-4103 0022-538X/88/114096-08$02.00/0 Copyright C 1988, American Society for Microbiology Characterization of Major Recognition Sequences for a Herpes Simplex Virus Type 1 Origin-Bind[r] ... See full document
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Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.
... Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oriS replication origin.. Genetic analysis of temperature-sensitive mutants w[r] ... See full document
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