[PDF] Top 20 Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.
Has 10000 "Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus." found on our website. Below are the top 20 most common "Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.".
Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.
... I7 protein. The thermosensitivity of VV ts16 has been mapped to the I7 gene, which encodes a 47-kDa polypeptide of yet-unknown function. The I7 protein is synthesized late in infection at both the permissive and ... See full document
15
Characterization of a temperature-sensitive mutant of vaccinia virus reveals a novel function that prevents virus-induced breakdown of RNA.
... At 40°C, ts22 showed a pattern of viral protein synthesis indistinguishable from that observed in WT-infected cells until 8 h postinfection, including host shutoff, synthesis and shutoff[r] ... See full document
9
Isolation and preliminary characterization of a phosphonoacetic acid-resistant and temperature-sensitive mutant of herpes simplex virus type 1.
... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document
10
Analysis of Murine Hepatitis Virus Strain A59 Temperature-Sensitive Mutant TS-LA6 Suggests that nsp10 Plays a Critical Role in Polyprotein Processing
... the TS mutant was engineered into the MHV clone, which is comprised of seven fragments main- tained in plasmids amplified and assembled according to previously described protocols (60) ...for 16 to ... See full document
13
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... A temperature-sensitive (ts) human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) mutant was generated by charged-cluster-to-alanine ...The mutant virus, ... See full document
8
Clustered Charge-to-Alanine Mutagenesis of the Vaccinia Virus H5 Gene: Isolation of a Dominant, Temperature-Sensitive Mutant with a Profound Defect in Morphogenesis
... The vaccinia virus H5 gene encodes a ...a temperature-sensitive (ts) virus with a lesion in the H5 ...Five mutant viruses were isolated, with one of them, tsH5-4, having a ... See full document
13
Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.
... conditional mutant viruses which would be useful for identification of amino acids required for the various functions of integrase during the course of infection and possibly for identification of residues ... See full document
5
Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins.
... It synthesi Ls, L cells were infected with either wt virus, ts2, or seemed plausible that the mutant Bi proteins might be more ts25 at ain MOI of 15 PFU per cell and maintained at 32 or [r] ... See full document
14
Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus.
... In the present growth of wild-type RS virus, ts-1, ts-2, and ts-7 incubated for 24 h at permissive or restrictive study this was confirmed when it was shown temperature that at least 13 [r] ... See full document
5
Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase.
... ts5 and wildtype virus ts' grown in MDCK cells at 320C were purified by sucrose gradient centrifugation and subjected to SDS-10% PAGE At the nonpermissive temperature, ts5 showed neither[r] ... See full document
10
Role of Vaccinia Virus A20R Protein in DNA Replication: Construction and Characterization of Temperature-Sensitive Mutants
... Overlapping PCR was used to mutate charged amino acids in the A20R ORF to alanines. A first PCR product was made with the U primer shown above and a primer that contained the mutations D-A20-x), in which x is the first ... See full document
8
Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
... The present study demonstrates that a molecular form of T antigen induced by ts 900, a transformation-defective ts mutant of SV40 29, is more heat labile than that induced by WT virus, i[r] ... See full document
6
Temperature-Sensitive Simian Virus 40 Mutant Defective in a Late Function
... To determine whether mutant SV40tsTNG-1 also induced cellular DNA synthesis, 5-day-old cultures of primary mouse kidney cells 3.3 X 106 cells per culture were infected at input multiplic[r] ... See full document
9
Further characterization of the vesicular stomatitis virus temperature-sensitive O45 mutant: intracellular conversion of the glycoprotein to a soluble form.
... 210 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Reexamination of the viral products of ts045, a glycoprotein mutant of vesicular stomatitis virus, showed that at 39[r] ... See full document
6
Control of protein synthesis by a temperature-sensitive mutant of reovirus 3. I. Temperature-sensitive function of ts261-b mutant.
... The protein-synthesizing ability of the Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest 40 IKEGAMI of in vivo synthesis at 370C of the mutant virusspecific ss RNAs of t[r] ... See full document
16
Temperature-sensitive vaccinia virus mutants identify a gene with an essential role in viral replication.
... The 24-kDa gene mapped downstream of the sequences encoding the 30-kDa polypeptide; the mRNA was hybrid selected by the XbaI 700- and 600-bp fragments lanes 11 and 12 and the adjacent Xb[r] ... See full document
10
Intramolecular homologous recombination in cells infected with temperature-sensitive mutants of vaccinia virus.
... In order to delineate the viral gene products which participate in vaccinia virus recombination, I monitored intramolecular homologous recombination within a plasmid in cells infected wi[r] ... See full document
6
Biochemical characterization of temperature-sensitive rabies virus mutants.
... Production of viral complementary RNA in cells infected by wild-type rabies virus at 39.60C in the presence or absence of cycloheximide.. Total unlabeled RNAs were extracted from rabies-[r] ... See full document
11
Isolation and characterization of temperature-sensitive mutants of measles virus.
... Other experiments data not shown have indicated that cells infected with ts-8 or ts-9 at 39 C reproducibly yield significant amounts of virus-specific RNA, but the amount of RNA synthesi[r] ... See full document
11
Temperature-Sensitive Transformation by an Abelson Virus Mutant Encoding an Altered SH2 Domain
... leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transfor- mation of immortalized fibroblast lines and pre-B ...cells. Temperature-sensitive mutations affecting the ... See full document
8
Related subjects