[PDF] Top 20 DNA Cleavage and Packaging Proteins Encoded by Genes UL28, UL15, and UL33 of Herpes Simplex Virus Type 1 Form a Complex in Infected Cells
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DNA Cleavage and Packaging Proteins Encoded by Genes UL28, UL15, and UL33 of Herpes Simplex Virus Type 1 Form a Complex in Infected Cells
... a 1-ml volume of buffer consisting of PBS containing 1% Tween 20 ...(PBSTw). Cells were then clarified in an Eppendorf Microfuge for 20 min at 14,000 ⫻ g, and the supernatants were transferred to new ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... in cells infected with wild-type virus and a virus with a mutant version of cleav- age and packaging gene UL6, capsids localize exclusively to replication compartments, ... See full document
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Linker Insertion Mutations in the Herpes Simplex Virus Type 1 UL28 Gene: Effects on UL28 Interaction with UL15 and UL33 and Identification of a Second-Site Mutation in the UL15 Gene That Suppresses a Lethal UL28 Mutation
... The UL28 protein of herpes simplex virus type 1 (HSV-1) is one of seven viral proteins required for the cleavage and packaging of viral ...that ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... Sf cells coinfected with recombinant baculoviruses expressing wt (AcUL33 virus) or mutated (Acin14 to Acin116) forms of UL33 and with AcUL28Myc, expressing a c-Myc-tagged form of ...that ... See full document
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Herpes Simplex Virus Type 1 Portal Protein UL6 Interacts with the Putative Terminase Subunits UL15 and UL28
... Herpes simplex virus type 1 (HSV-1) DNA is packaged into a preassembled icosahedral capsid, referred to as the procap- sid, within the cell nucleus (28, 29, ...viral ... See full document
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Herpes Simplex Virus DNA Cleavage and Packaging: Association of Multiple Forms of UL15-Encoded Proteins with B Capsids Requires at Least the UL6, UL17, and UL28 Genes
... nuclei. Cleavage of ICP35 in procapsids by the viral protease likely releases the scaffold from the inner side of the outer capsid shell, allowing the scaffold to collapse inward to produce small-cored B capsids ... See full document
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The pseudorabies virus UL28 protein enters the nucleus after coexpression with the herpes simplex virus UL15 protein.
... all UL28-null and UL28-temperature- sensitive mutants is a cessation of viral replication at the point of DNA cleavage and encapsidation (1, 6, 14, 17, ...of UL28 is to act in ... See full document
6
Characterization of ICP6::lacZ insertion mutants of the UL15 gene of herpes simplex virus type 1 reveals the translation of two proteins.
... The herpes simplex virus type 1 (HSV-1) UL15 gene is a spliced gene composed of two exons and is predicted to encode an 81-kDa protein of 735 amino acids ...Two ... See full document
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Herpes Simplex Virus DNA Cleavage and Packaging Proteins Associate with the Procapsid prior to Its Maturation
... of DNA into preformed capsids is a fundamental early event in the assembly of herpes simplex virus type 1 (HSV-1) ...viral DNA genomes, in the form of ... See full document
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Herpes Simplex Virus Type 1 Cleavage and Packaging Proteins UL15 and UL28 Are Associated with B but Not C Capsids during Packaging
... HSV-1 cleavage and packaging proteins in procapsids and ma- ture virions may provide clues as to their possible ...HSV-1 DNA cleavage and pack- aging proteins, are ... See full document
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Herpes Simplex Virus 1 DNA Packaging Proteins Encoded by UL6, UL15, UL17, UL28, and UL33 Are Located on the External Surface of the Viral Capsid
... Capsids form in the nuclei of cells infected with all herpes- ...viruses. Herpes simplex virus (HSV) capsid pentons and hex- ons form spontaneously from five and ... See full document
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Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.
... and 10% vol/vol tryptose phosphate broth Difco on 314 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sensitive mutants of herpes simplex virus type 1 repre[r] ... See full document
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Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication.
... 7Zf 1 (Promega, Madison, ...pBluescript 1 (Stratagene, La Jolla, ...pGEM-3Zf 1 (Promega) to generate ...helicase-primase complex genes fused to short defined peptide epitopes from the ... See full document
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Association between the p170 form of human topoisomerase II and progeny viral DNA in cells infected with herpes simplex virus type 1.
... In the present study, we show that topoisomerase II activity persists in HSV-1-infected cells and that both forms are present throughout the infection; however, only p170 topoisomerase I[r] ... See full document
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Identification and characterization of a DNA primase activity present in herpes simplex virus type 1-infected HeLa cells.
... Percentage of DNA primase activity remaining in solution after treatment with anti-calf peptide IgGs and after binding of the antibody-antigen complex to protein A-Sepharose" Enzyme % Ac[r] ... See full document
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DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells.
... First, mock-infected cell DNA-binding proteins were concentrated from 0.1, 0.3, 0.5, and 1.0 M NaCl eluates from a denatured DNA-cellulose column and subjected to electrophoresis in adja[r] ... See full document
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Mutations in herpes simplex virus type 1 genes encoding VP5 and VP23 abrogate capsid formation and cleavage of replicated DNA.
... The capsid gene products are essential for virion morphogenesis, and therefore a prerequisite for the isolation of null mutants for these genes is construction of transformed cell lines [r] ... See full document
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Topoisomerase II cleavage of herpes simplex virus type 1 DNA in vivo is replication dependent.
... Figure 8 summarizes the cleavages mapped in vivo in this DISCUSSION Topoisomerase II consensus sequences are distributed throughout the HSV-1 genome, and some of these sites are recogniz[r] ... See full document
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Identification of proteins encoded by a fragment of herpes simplex virus type 2 DNA that has transforming activity.
... Our preliminary analysis of late mRNAs encoded by two other regions of the HSV-2 genome, represented by the viral insertions in pDG304 and pDG305 corresponding to BglII fragments J 0.32 [r] ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... (residues 1 to 45), to be the least conserved portion of the protein ...corresponding proteins of the other human alphaherpesviruses, ...varicella-zoster virus (HHV-3), which exhibited similari- ties ... See full document
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