[PDF] Top 20 Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... Serotyping dengue virus (DENV) from suspect human specimens is crucial for developing sound epidemi- ological control measurements early in the transmission season and for effective patient ...and ... See full document
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... from viruses of known ...two real-time, one-tube, multiplex TaqMan assays were then used to detect RNAs extracted from the laboratory-reared, virus-inoculated ... See full document
6
Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... genetic assays. Two real-time reverse transcriptase PCR (RT-PCR) assays performed in a LightCycler instrument were developed and compared regarding specificity and ... See full document
6
Development of One Step, Real Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
... erally time-consuming and therefore have little clinical signif- ...as reverse transcriptase PCR (RT-PCR) (15), nested RT-PCR (11), and, recently, also real-time ... See full document
5
Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single Tube Multiplex Reverse Transcriptase PCR
... years, dengue viruses (serotypes 1 to 4) have spread throughout tropical regions ...multiple dengue virus serotypes are circulating concurrently, which may increase the risk for the more severe form ... See full document
6
Development of a pan Simbu real time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
... We thank Christian Korthase, Anja Landmesser, Karin Lissek and Patrick Zitzow for excellent technical assistance as well as Dr. Dirk Höper and Dr. Katja Goller for providing sequence information. Simbu serogroup ... See full document
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Duplex Real Time Reverse Transcriptase PCR Assays for Rapid Detection and Identification of Pandemic (H1N1) 2009 and Seasonal Influenza A/H1, A/H3, and B Viruses
... A real-time reverse transcriptase PCR (RT-PCR) assay for the detection of pandemic (H1N1) 2009 was designed and used with existing real-time RT-PCR ... See full document
5
Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... with assays B, C, and D compared with assay A in the present ...used real-time RT-PCR assay for HEV detection (assay A) (17), and a modification of the probe and subsequent increase of the ... See full document
7
Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... two PCR assays, both detected the same samples (n = 14) as CHPV-RNA ...of real-time assay was ...of real time PCR for Epstein- Barr virus (detection level = 100 copies/ml) ... See full document
7
Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... the dengue group- and serotype-specific primer pairs can be used to detect and differentiate the majority strains of four dengue virus serotypes, we had selected and tested 16 dengue ... See full document
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... Assays for the detection of RNA viruses are often highly complex (high quantity of different oligonucleo- tides) due to low sequence conservation of the RNA genome (Gardner et al. 2004; Brownie et al. ... See full document
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... tiplex PCR differs depending on the template ...from viruses grown in cell culture or from clinical samples was used as a template, the SYBR green RT- PCR displayed equal or higher sensitivity than ... See full document
7
Development of real time reverse transcriptase qPCR assays for the detection of Punta Toro virus and Pichinde virus
... of PCR are found in complex matrices such as blood [2] or stool [23, 28], and these inhibitors can carry through RNA preparation methods, affecting real-time RT-qPCR results and impacting assay ... See full document
6
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... TaqMan real-time RT-PCR assay was shown to be extremely sensitive for the detection of influenza A H5 viruses, detecting clade 1, 2, and 3 H5 virus levels of 1 PFU or less by using a ... See full document
9
Development and Evaluation of Serotype and Group Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus
... of dengue virus. These assays have several advantages over traditional identifi- cation ...RT-PCR assays are more rapid than traditional methods. The TaqMan RT-PCR assays take ... See full document
6
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... multiplex real-time RT-PCR assay. A real-time, one-step, multiplex RT-PCR assay was developed for the detection and quantitation of DENV and CHIKV RNAs in cell culture ... See full document
8
Development of an Internally Controlled Real Time Reverse Transcriptase PCR Assay for Pan Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays
... A further benefit of the pan-DENV assay is the multiplex de- tection of RNase P, which serves as a heterologous intrinsic IC. Of the method comparisons mentioned previously, only the conven- tional RT-PCR ... See full document
10
Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... a real-time reverse transcrip- tase PCR assay for the detection of EV-D68 in clinical ...The development of this assay was informed by sequencing the complete genome of the EV-D68 virus ... See full document
7
Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... (DEN) viruses are a group of four closely related arboviruses in the genus Flavivirus, designated DEN-1, DEN-2, DEN-3, and DEN-4 viruses ...DEN viruses, and humans are the primary ...DEN ... See full document
7
Development of real time PCR for detection and quantitation of Dengue Viruses
... qRT- PCR. The transcribed or viral RNA was reverse transcribed using the reverse primer with AMV reverse transcriptase (Promega Corporation, Madison, WI, ...The reverse ... See full document
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