[PDF] Top 20 Up to Four Distinct Polypeptides Are Produced from the γ34.5 Open Reading Frame of Herpes Simplex Virus 2
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Up to Four Distinct Polypeptides Are Produced from the γ34.5 Open Reading Frame of Herpes Simplex Virus 2
... isolated from cells 6 h postinfection, when robust expression of C1 and C2 is ...the 5= untrans- lated region was paired with a series of reverse primers to walk along the entire ...RT-PCR from both ... See full document
13
Varicella-zoster virus open reading frame 4 encodes a transcriptional activator that is functionally distinct from that of herpes simplex virus homology ICP27.
... First, it is possible that the upstream elements merely function to boost the basal transcriptional activity of the promoter and that activation by the ORF4 gene product is not discerned[r] ... See full document
10
Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... FIG. 5. Amino acid sequence alignment of the U L 32 homologs in equine herpes virus (EHV) (51), varicella-zoster virus (VZV) (11), and HSV-1 ...harvested from a flotation sucrose ... See full document
9
Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27.
... Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a putative immediate-early protein which is homologous to herpes simplex virus type 1 (HSV-1) ICP27 on the basis of gene [r] ... See full document
6
Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
... formed similar rods, but usually nuclei contained a single, more compact, rod-like structure. Also seen clearly with poly- clonal antisera was a second set of bands that migrated signif- icantly more slowly than did the ... See full document
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The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.
... The results shown in Fig. 9 are as follows. In some cells exem- plified by the cell in the panel A, the distribution of ORF P antigen is asymmetric, and because of the large amount of the protein, it is difficult to ... See full document
9
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene.
... One site of nucleotidylylation ascribed to casein kinase II is in the amino-terminal domain between amino acids 78 and 81 (4, 25). The precise location of the sequences phosphorylated by the U S 3 and U L 13 protein ... See full document
7
The Herpes Simplex Virus Type 1 UL3 Transcript Starts within the UL3 Open Reading Frame and Encodes a 224-Amino-Acid Protein
... 2007/Accepted 2 July 2007 Several different herpes simplex viruses (HSVs) and vectors are being explored as therapeutic products for use in the treatment of cancer and neurological ...differ ... See full document
8
The Genome Sequence of Herpes Simplex Virus Type 2
... are distinct in their ...differences from the mean diminish sharply with increasing size of open reading frames (ORFs), suggesting pronounced stochastic effects, especially with the values for ... See full document
12
Transcription of the derepressed open reading frame P of herpes simplex virus 1 precludes the expression of the antisense gamma(1)34.5 gene and may account for the attenuation of the mutant virus.
... derived from these results is that accumulations of detectable amounts of the two antisense mR- NAs, those of ORF P and g 1 ...extend 5 9 of the g 1 ...) 2 R indicates that the control of g 1 ... See full document
8
Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ... See full document
12
Herpes Simplex Virus γ34 5 Interferes with Autophagosome Maturation and Antigen Presentation in Dendritic Cells
... an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike ...erpes simplex virus 1 (HSV-1) is a common and significant pathogen with two ... See full document
9
Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1.
... In this series of experiments, replicate 25-cm2 cultures of Vero cells were infected in duplicate with the parent, mutant, or rescued virus (5 PFU per cell) and incubated in a 34°C incub[r] ... See full document
7
Physical mapping of herpes simplex virus-induced polypeptides.
... Control of protein synthesis in herpes- virus infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. The location o[r] ... See full document
19
Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
... Elution of HSV-1-infected cell proteins from single-stranded DNA agarose columns by using a linear KCI gradient demonstrated that the UL37 protein elutes from this matrix at a salt conce[r] ... See full document
9
The herpes simplex virus Us11 open reading frame encodes a sequence-specific RNA-binding protein.
... Specifically, (i) host protein synthesis is decreased grossly late in infection by a viral function other than that of the vhs gene (encoded by the UL41 open reading frame), which degrad[r] ... See full document
8
Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.
... gave identical tryptic methionine peptide profiles for the major capsid polypeptides1. Trypsinization and ion- exchange chromatography on Chromobeads P (Tech- nicon) were carried out acc[r] ... See full document
11
Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ... See full document
11
The herpes simplex virus origins of DNA synthesis in the S component are each contained in a transcribed open reading frame.
... In the herpes simplex virus 1 genome, the origins of viral DNA synthesis are located in the unique sequences of the L component (Ori) and in the reiterated sequences of the S component ([r] ... See full document
7
Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0.
... To investigate the functional relatedness of HSV-1 ICPO and VZV ORF61 protein, we established Vero and MeWo cell lines which stably express VZV ORF61 under the control of a metallothione[r] ... See full document
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