[PDF] Top 20 Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... appropriate reference genes are fundamental to get reliable results from ...thirty-nine genes annotated as 18S rRNA (18S ribosomal RNA), CYP (cytochrome), Actin, GAPDH ... See full document
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Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in Plutella xylostella (Lepidoptera: Plutellidae)
... appropriate reference genes with stable expression across various biotic and abiotic ...didate genes from ...for normalization of gene expression in ...housekeeping genes [ACTB, GAPDH, ... See full document
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Selection of reference genes for quantitative real time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera
... candidate reference genes were evaluated for gene expression stability by qRT-PCR, using qRT-PCR primers designed in exonic regions from the cloned and sequenced Boechera homologues (Table ... See full document
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Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)
... from real-time PCR analysis, accurate normalization of gene expression against a reference gene is ...The reference gene should have stable expression independent of the ... See full document
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Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR
... Green real-time qPCR has been used for analysis of copy number variation in both the α- and β-globin gene clusters ...relative, quantitative PCR method using SYBR Green ...both time and ... See full document
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Accurate normalization of real time quantitative RT PCR data by geometric averaging of multiple internal control genes
... control genes as determined by geNorm are based on only two RNA samples (that is, a Cy3-labeled refer- ence pool, and a Cy5-labeled test sample), in contrast to the RT-PCR data, where 9 to 34 samples were ... See full document
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Genomic selection of reference genes for real-time PCR in human myocardium
... both genes tended to be lower in failed myocardium compared with non-failed myocar- dium, TPT1 and EEF1A1 require further validation in a larger sample to confirm that their expression levels do not differ between ... See full document
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Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
... suppressor genes lose their function ...validate reference genes in the particular experimental system in order to trust the differential gene expressions which are ...stable reference ... See full document
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Validation of reference genes for expression analysis by quantitative real time PCR in Leptinotarsa decemlineata (Say)
... house-keeping genes (Actin, ACT1 and ACT2; ADP- ribosylation factor, ARF1 and ARF4; TATA box binding protein, TBP1 and TBP2; ribosomal protein RP4 and RP18; translation elongation factor 1 α EF1 α ) was evaluated ... See full document
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... identify genes that were the most stably expressed for all of the experimental ...control genes) and V (the pairwise variation).The least stable genes have the highest M values and are successively ... See full document
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Microarray based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress
... The identification of suitable reference genes can be ...“model” genes to be used in data normalisation. Usually the allocated genes vary with the plant species, as well with the ... See full document
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Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR
... these reference genes have constant level of expression in different tissues and under different treatments and has no inter-individual variability ...ideal reference gene for normalisa- tion in ... See full document
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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
... ideal reference controls for normalizing candidate biomarkers, approximately ten miRNAs with the least fold-changes are also detected in all ...the normalization con- ... See full document
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Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures
... as reference genes, also in neurobiological ...sical reference genes may vary per tissue type and developmental stage, and may even vary in response to ex- perimental treatment ...suitable ... See full document
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Selection of Reference Genes in Equine White Blood Cells for Real Time PCR Normalization Following Extracorporeal Shock Wave Therapy
... The candidate RGs analyzed in present study are generally used as internal controls, because they are consi- dered to have relatively stable expression in many tissues. However, examples exist to demonstrate that this ... See full document
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Selection of reference genes for quantitative real time PCR in equine in vivo and fresh and frozen thawed in vitro blastocysts
... in normalization to internal reference genes, which should be constitutively expressed without influence of the experimental ...universal reference gene with a constant expression in all ... See full document
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Selection of reliable reference genes for quantitative real time PCR in human T cells and neutrophils
... instable reference genes correctly indicated an increase in IL-2 transcripts, which may be sufficient if only an on-/off response is to be ...inappropriate reference genes leads to unreliable ... See full document
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Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)
... 14 genes were less than ...candidate reference genes were sta- ble. As one of the least stable genes, the instability of APT has been reported in papaya under six experimental con- ditions ... See full document
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Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage
... housekeeping genes are combined together to form the BestKeeper ...of genes in terms of their ...ing genes, the authors recommended the use of the best 3 or 4 genes as that provides a ... See full document
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Identification of stable reference genes for quantitative PCR in koalas
... Quantitative real time PCR has been used widely in molecular biology research to evaluate gene expression under certain ...data, normalization of gene expression with a reference ... See full document
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