[PDF] Top 20 Isolation and Characterization of the Herpes Simplex Virus 1 Terminase Complex

... During herpes simplex virus 1 (HSV-1) infection, empty procapsids are assembled and subsequently filled with the viral genome by means of a protein complex called the ... See full document

... The biochemical approach of isolating primary enveloped HSV-1 virions complements the analysis of this assembly in- termediate by electron and fluorescent microscopy. After con- firming the efficiency of the ... See full document

... Vero cells were biochemically transformed with the gene encoding ICP4 of herpes simplex virus type 2 (HSV-2).. These cells were used as permissive hosts to isolate and propagate HSV-2 mu[r] ... See full document

... Our find- ing that the PAA15tsl and PAArtsl + recombi- nant HSV-1 mutants synthesized normal levels of viral DNA at 39.50C, while still being defec- tive in shutdown of host DNA synthesi[r] ... See full document

... All three mutants (hr27, hr48, and hr156) were defective in the synthesis of viral DNA and late proteins when grown in nonpermissive Vero cells.. Early gene expression in cells infected [r] ... See full document

... We have derived Vero cell lines containing the herpes simplex virus DNA polymerase (pol) gene that complement temperature-sensitive pol mutants.. These cell lines were used to recover vi[r] ... See full document

... The UL42 deletion mutant, designated CgalA42, displayed a tight early phenotype in nonpermissive Vero cells producing no infectious progeny, viral DNA, or late gene products but accumula[r] ... See full document

... Further characteri- zation was achieved by amino acid analysis, determination of the N-terminal amino acid sequence in a gas-phase sequencer and by reaction with monoclonal antibodies di[r] ... See full document

... The fact that early and late polypeptides were expressed relatively inefficiently in ICP4- transformed cells infected with the ts mutant, whereas those of the deletion mutants were effic[r] ... See full document

... Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characteri- zation of an ICP6 lacZ insertion mutant[r] ... See full document

... Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome. Isolation and translation of mRNA encoded by a specific region o[r] ... See full document

... biochemical characterization of VP22, and any complete understanding of the role and de- tailed mechanisms of assembly and involvement in virus-host interactions will require such analyses in addition to ... See full document

... and 1% arabinose was added to induce the expression of the SceI restriction ...for 1 h at 32°C with shaking, the Red recombination system was induced by incubation in a 42°C water bath with shaking for 30 ... See full document

... fied nuclear extract through a molecular sieve matrix yielded a single peak of immunoreactive ICP4 that remained soluble at ionic strengths as low as 50 mM.. Details of the purification [r] ... See full document

... The size distributions of viral mRNA encoded by differ- ent regions of the HSV-1 genome also have been determined by first fractionating infected-cell mRNA on denaturing agarose gels and[r] ... See full document

... The herpes simplex virus type 1 (HSV-1) UL6, UL15, and UL28 proteins are essential for cleavage of replicated concatemeric viral DNA into unit length genomes and their packaging into a ... See full document

... picture of the protein composition of the virions, viral prepa- rations from HeLa and BHK cells were compared by Western blotting with the Remus polyclonal HSV-1 antibody and by silver staining. Figure 7B shows ... See full document

... An immunoperoxidase procedure was employed to study the expression of a large-molecular-weight, virus-induced polypeptide (VP175; molecular weight, 175,000) at the light and electron mic[r] ... See full document

... Map locations of the IE mRNA's contained in resolved fractions 1, 3, and 5 (the 4.7-, 3.0-, and 2.0-kb RNAs, respectively) are compared to those of unresolved cytoplasmic poly(A) IE mRNA[r] ... See full document

... putative terminase subunits of herpes simplex virus 1 (HSV-1) encoded by U L 15 and U L 28 (designated pU L 15 and pU L 28, respectively) coimmunoprecipitate with the U L 33 ... See full document