[PDF] Top 20 Multiplex PCR detection of vanA, vanB, vanC 1, and vanC 2/3 genes in enterococci
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Multiplex PCR detection of vanA, vanB, vanC 1, and vanC 2/3 genes in enterococci
... Our study confirms the work of Dutka-Malen and colleagues (5), who used a multiplex PCR to detect glycopeptide resis- tance genes in Enterococcus spp.; however, our study distin- guishes itself in ... See full document
5
Comparison of the Roche LightCycler vanA/vanB Detection Assay and Culture for Detection of Vancomycin Resistant Enterococci from Perianal Swabs
... developed PCR assays for the di- rect detection of van genes from rectal or perirectal ...a multiplex PCR assay to detect vanA, vanB, vanC-1, and ... See full document
8
Development of a Multiplex PCR for the Detection of asa1, gelE, cylA, esp, and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital Isolates of Enterococcus faecium
... A multiplex PCR for the simultaneous detection of five virulence genes (asa1, gelE, cylA, esp, and hyl) in enterococci was ...these genes was investigated in 153 clinical and 118 ... See full document
7
Clinical and epidemiological significance of enterococci intrinsically resistant to vancomycin (possessing the vanC genotype)
... growth. PCR-based detection of vancomycin resistance ...A multiplex PCR assay was used to detect genes coding for vancomycin resistance in ...the vanA ligase gene was amplified ... See full document
6
Validation of VITEK 2 Version 4 01 Software for Detection, Identification, and Classification of Glycopeptide Resistant Enterococci
... the detection of ...the detection of enterococci harboring the vanB, vanC1, or vanC2 gene (7, 11, ...VITEK 2 system correctly detected all vanA-carrying resistant enterocooci but ... See full document
6
Evaluation of the Xpert vanA/vanB Assay Using Enriched Inoculated Broths for Direct Detection of vanB Vancomycin Resistant Enterococci
... accurate detection of VRE is required for adequate antimicrobial treatment and infection prevention ...evaluated PCR-based meth- ods for rapid detection of VRE, including the Cepheid Xpert ... See full document
5
Molecular detection of <em>vanA</em> and <em>vanB</em> genes among vancomycin-resistant enterococci in ICU-hospitalized patients in Ahvaz in southwest of Iran
... of enterococci to 10 antibiotics were determined based on the Clinical and Laboratory Standards Institute ...the vanA and vanB genes in vancomycin-resistant isolates were amplified by ... See full document
7
Bacteriocin activity of enterococci and presence of genes related to pathogenesis
... 228 enterococci strains isolated from food were ...monocytogenes. Multiplex PCR for the detection of seven virulence genes in bacteriocin producing strains showed that ...carried ... See full document
6
Detection of vancomycin resistant enterococci in fecal samples by PCR
... our PCR assays for VRE were compared with the results of culture tests with several different selective media with and without the aid of broth ...of vanA-con- taining enterococci from rectal and ... See full document
6
Characterization of an Enterococcus gallinarum Isolate Carrying a Dual vanA and vanB Cassette
... vanC1, vanA, and vanB genes by PCR. Considering that vanA and vanB clusters are commonly acquired through mobile, conjugative elements, the plasmid DNA from the isolate was ... See full document
5
Rapid characterization schemes for surveillance isolates of vancomycin resistant enterococci
... vancomycin-resistant enterococci (VRE) and subsequent characterization of the isolates can be extremely burdensome and ...and PCR-based schemes for the detection and characterization of ...the ... See full document
5
The Antibiotic Susceptibility Pattern and Prevalence of vanA, vanB, and vanC Genes among Enterococcus faecalis Strains Isolated from Consumed Meat
... antibiotic-resistant genes transferred to other species, their permanent existence in the human and animal gut as normal flora, their clinical significance in causing infections, their ability to acquire ... See full document
5
Detection of virulence genes in Malaysian Shigellaspecies by multiplex PCR assay
... Virulence genes responsible for the pathogenesis of shig- ellosis may be located in the chromosome or on the inv plasmid borne by the ...the genes tend to be clustered in the genome. Previously reported ... See full document
7
Molecular Analysis of Glycopeptide ResistantEnterococcus faecium Isolates Collected from Michigan Hospitals over a 6 Year Period
... a vanB isolate between three hospitals in two states ...the vanA gene from the northeastern United States differs from strains initially re- ported from Europe ...multiresistant VanA isolates ...that ... See full document
6
Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
... the multiplex PCR was the ability to amplify all PCR groups by using either a 55 or 60°C annealing ...Group 2 species primers were the most sensitive to changes in annealing temperature and ... See full document
8
Kinetics of Poliovirus Shedding following Oral Vaccination as Measured by Quantitative Reverse Transcription PCR versus Culture
... It may be particularly important to identify long-duration or high-frequency shedders since they appear to contribute dispro- portionately to the OPV reservoir. Indeed, we found by qRT-PCR that a small minority of ... See full document
6
Detection and Identification of Actinobacillus pleuropneumoniae Serotypes 1, 2, and 8 by Multiplex PCR
... serotypes 1 and 2 were previously de- termined (reference 24 and unpublished ...5 multiplex PCR assay to include serotypes 1, 2, and ... See full document
5
Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of Streptococcus pneumoniae in Purulent Sputum Samples by Use of a Novel Real Time Multiplex PCR Assay
... resistance genes in other micro- organisms in the sputum ...mosaic genes that encode altered, low-affinity pbp genes are considered products of recombination events involving horizontal transfer from ... See full document
5
Multiplex PCR and Oligonucleotide Microarray for Detection of Single Nucleotide Polymorphisms Associated with Plasmodium falciparum Drug Resistance
... Table 1. mPCR was performed with the PCR amplification kit of Genscript (Piscataway, NJ) accord- ing to the manufacturer’s ...of 2⫻ Genscript master mix, and ...for 1 cycle; followed by 30 ... See full document
8
Detection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
... for detection of CDI. We aimed to use a multiplex PCR to detect the ...toxin genes tcdA, tcdB and tpi in stool of cancerous ...tpi genes were ...the detection rate of A - B + ... See full document
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