[PDF] Top 20 Population Structure and Properties of Candida albicans, as Determined by Multilocus Sequence Typing

... definition of a clade was therefore used: a cluster of at least five isolates with a dissimilarity not exceeding 10 by pairwise-difference analysis. By this definition, 348 (83.7%) of the 416 isolates could be assigned ... See full document

... 11, Candida species recovered from peri- odontal pockets were not recovered by oral rinse sampling of the same patient or by the sampling of an alternative periodontal pocket (Table 2), indicating site-specific ... See full document

... phenotypic properties in the fungus, including sorbose utiliza- tion and fluoroorotic acid resistance (26, 94) and the genera- tion of MTL-homozygous variants ...C. albicans evidenced in our data, including ... See full document

... C. albicans isolates with the same sequences for six of the seven MLST genes but with the seventh gene in one isolate containing, say, seven heterozygous SNPs while the other isolate has no heterozygous SNPs in ... See full document

... Multilocus sequence typing (MLST) is a molecular typing method used to characterize strains in terms of their pathogenicity and can be applied to Borreliella iso- ...discriminating ... See full document

... investigate Candida isolates recovered from periodontitis patients by MLST, which is highly reproducible and reliable in determinating the genetic relatedness between isolates of the same ...the Candida ... See full document

... molecular typing approach has certainly become more necessary in the health care environment for addressing reported nosocomial ...C. albicans MLST ...relationships determined by use of the other ... See full document

... between Candida carriage in the digestive tract and the presence of ASCA and other genetic and environ- mental factors associated with CD (12, ...CHROMagar Candida medium (Bio-Me ´rieux, Marcy-l’Etoile, ... See full document

... combining multilocus sequence typing with molecular evolutionary analysis determined the distribution, population structure, and evolution of antibiotic resistance in Neisseria ... See full document

... was determined by chromogenic detection of penicillinase produc- tion (Cefinase; bioMérieux) and by the agar disk diffusion method ac- cording to the recommendations of the Comité de l’Antibiogramme de la Société ... See full document

... the population structure of ...of sequence change also suggests that this typing technique may not be ideal for local epi- demiological studies [6, ... See full document

... A multilocus sequence typing (MLST) scheme was developed to study the genetic relationships and popu- lation structure of 72 Clostridium difficile isolates from various hosts, geographic ... See full document

... yeast Candida glabrata is the second most common Candida species isolated from cases of bloodstream ...or population structure of this species. We developed a multilocus sequence ... See full document

... 51 Candida albicans clinical isolates and 2 reference strains were used in this ...C. albicans clinical isolates can be divided into 3 ...C. albicans isolates were determined by the ... See full document

... strain typing have been developed for this species (32), but no system has yet achieved universal ...C. albicans nucleotide sequences show greater or lesser diversity of types depending on the extent of ... See full document

... C. albicans isolates that we studied in this work were specifically chosen to constitute a sampling of strains, with some having specific epidemiological links and some being ...C. albicans by using a ... See full document

... MLP typing system, we allocated one MLST group for each distinct group of MLP, corresponding to the greater number of isolates in the MLP group belonging to a MLST ...group, determined the false-negative ... See full document

... The performance data reported in this study are comparable to those previously reported for C. albicans PNA FISH as well as other PNA FISH assays for other blood pathogens (13, 19). PNA FISH has proven to be ... See full document

... The mutS-nlpD region represents a major operational region of genomic evolution in Enterobacteriaceae. Pre- vious studies suggested that the polymorphic nature of this genetic region due to high mutation rate and to loss ... See full document

... MLST analysis of in vitro-isolated H. suis strains and H. suis strains present in porcine and human gastric tissue. Based on the complete gene sequences, as described above, internal gene fragments/variability regions ... See full document