[PDF] Top 20 RNA isolation method for single embryo transcriptome analysis in zebrafish

... the method was applicable to all stages of zebrafish embryogenesis; the success rate was almost 100%; and the extracted RNA performed excellent in microarray experiments in that the technical ... See full document

... fish embryo proteome. In addition, we also 777 performed transcriptome mining by RNA-seq to provide an 778 informative comparative map of the quanti fied proteome and 779 transcriptome during ... See full document

... SUPeR-seq method, a highly sensitive and reproducible method to simultaneously detect both poly(A)+ and poly(A)- RNA species within a single mam- malian ...this method, we analyzed a ... See full document

... from single-cell human embryo profiling studies [accession numbers: SRP011546 (Yan et ...two-pass method for novel splice detection (Engström et ... See full document

... the analysis of the function of Traf6 we could identify a gene set whose transcription is controlled by morpholino injections in ...the transcriptome level we provide a reference set for morpholino off- ... See full document

... network-based analysis approach were superior to their single-gene approach in terms of both statistical power and biological interpretability, A variety of interesting biological hypotheses were derived ... See full document

... study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole ...whole- transcriptome analysis of early embryos in flowering plants has been hampered by ... See full document

... ‘single-step’ method has become widely used for isolating total RNA from biological samples of different ...the method is that RNA is separated from DNA after extraction with an acidic ... See full document

... biochemical analysis in zebrafish embryo exposed to a single dose of thioacetamide and showed that the whole zebrafish embryo model is suitable to detect steatohepatitis 57 ... See full document

... new method of size selection would have greatly benefited attempts to produce libraries using chemical ...resulting single stranded cDNA would contain both adaptor sequences, PCR amplification could then ... See full document

... the embryo cells. Regardless, if we accept the premise that maternal RNA is cleared previous to the gas- trula stage, this means that, except for the identified 157 starting genes (type 9), all zygotic gene ... See full document

... qPCR analysis. cDNA was reverse transcribed from all cells of one embryo per gene repli- cate using the same protocol we used for single-cell RNA-seq 46,47 ...each embryo were scaled ... See full document

... efficient method for removal of 16S, 23S and, 5S ribosomal RNA from 100 ng to 5 μg of total RNA, in a single round of depletion. The system utilizes probe designs from highly conserved regions ... See full document

... isolate RNA with high quality and sufficient quantity from ...previous transcriptome studies, RNA of ...small RNA and other non-coding ...for single kind of RNA extraction and ... See full document

... These lines showed an almost identical pattern to each other and to the injected embryos. In opposition to that, CRE2 and CRE3 could drive tissue-specific expression, in the floor plate and in the vasculature ... See full document

... of RNA deep sequencing and proteomics have surprisingly high correlation if one considers the extreme differences between regulatory mechanisms at the level of transcription and transla- ...microarray ... See full document

... of zebrafish embryonic development, we employed next generation sequencing for full transcriptome analysis of normal embryos and embryos lacking overt DV ...promoter analysis of the validated ... See full document

... parative RNA-seq analysis of these three corepressor com- ponents indicates that approximately half of upregulated and downregulated genes were associated with the SIN-3–SPR-1 ... See full document

... using RNA-Seq ...using RNA-Seq ...to RNA-Seq data for cultured human B-cells derived from 27 unrelated individuals and 10 pairs of monozygotic twins in the Centre d’Etude du Polymorphisme Humain ... See full document

... the embryo and adult have similarities in the sequence of events underlying forma- tion of functional ...the embryo, neurons are derived from the neuroectodermal epithelium, which becomes structured in a ... See full document