[PDF] Top 20 Temperature-sensitive mutants of herpes simplex virus type 1 defective in lysis but not in transformation.
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Temperature-sensitive mutants of herpes simplex virus type 1 defective in lysis but not in transformation.
... Mutants representing all seven complementation groups failed to grow in either of these two transformed cell lines at the nonpermissive temperature, as measured by plaque formation on th[r] ... See full document
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Cysteine mutants of herpes simplex virus type 1 glycoprotein D exhibit temperature-sensitive properties in structure and function.
... When we analyzed each of the mutants in a complementation assay for virus function 38, only two mutant proteins, Cys-2 and Cys-4, Glycoprotein D gD of herpes simplex virus HSV is a struc[r] ... See full document
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Biochemical transformation by temperature-sensitive mutants of herpes simplex virus type 1.
... 704 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Biochemical transformation assays of herpes simplex virus type 1 temperaturesensitive ts mutants distinguished three[r] ... See full document
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Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein.
... 354-366 0022-538X/83/010354-13$02.00/0 Copyright C 1983, American Society for Microbiology Genetic Analysis of Temperature-Sensitive Mutants Which Define the Gene for the Major Herpes Si[r] ... See full document
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Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein
... Fig. 1, with all non-ts viruses (wt, tsRrev, tsSrev), temperature elevation from 33°C to 39°C had little influence on the final yields of viral ...the temperature was elevated to 39°C. With the tsOBP ... See full document
6
Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175.
... Consistent with this model are the following observations: i certain late proteins e.g., VP154 can be expressed in the absence of functional VP175 this study, ii VP175 is required for ce[r] ... See full document
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DNA-negative temperature-sensitive mutants of herpes simplex virus type 1: patterns of viral DNA synthesis after temperature shift-up.
... For each gradient, 45 0.1-ml fractions were collected ISCO density gradient fractionator, model 640; Instrumentation Specialties Co., Lincoln, Neb., refractive in- 1043 Downloaded from h[r] ... See full document
6
DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.
... The remaining mutants studied, all of which were DNA- at 39 C Table 1, fell into three categories with respect to their ability to induce DP activity at 39 C Table 2: i mutants belonging[r] ... See full document
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Electron microscope studies of temperature-sensitive mutants of herpes simplex virus type 2.
... For infectivity assay a 1.5-ml sample of infected cell suspension was frozen, thawed, and then sonicated for 1 min at 10 kcycles/s with a 727 Downloaded from http://jvi.asm.org/ on Novem[r] ... See full document
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Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.
... and 10% vol/vol tryptose phosphate broth Difco on 314 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sensitive mutants of herpes simplex virus type 1 repre[r] ... See full document
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Temperature-sensitive mutants in herpes simplex virus type 1 ICP4 permissive for early gene expression.
... Fifteen to fifty percent of wild-type levels of viral DNA are synthesized at the nonpermissive temperature in ts48- and ts303-infected cells, indicating that immediate-early and early ge[r] ... See full document
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Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants.
... In addition, survival rates determined from plating efficiencies of the various cell mutants after incubations at 39.5°C for the time periods corresponding 42 Downloaded from http://jvi.[r] ... See full document
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A Replication-Competent, Neuronal Spread-Defective, Live Attenuated Herpes Simplex Virus Type 1 Vaccine
... The GlaxoSmithKline HSV-2 gD subunit human vaccine trial showed partial protection in seronegative women but no protection in HSV-1-seropositive women or men (36). The gender difference in protection remains ... See full document
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Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1.
... In this series of experiments, replicate 25-cm2 cultures of Vero cells were infected in duplicate with the parent, mutant, or rescued virus 5 PFU per cell and incubated in a 34°C incubat[r] ... See full document
7
Isolation and preliminary characterization of a phosphonoacetic acid-resistant and temperature-sensitive mutant of herpes simplex virus type 1.
... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document
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Temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus.
... QUAGLIANA Department of Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 Received for publication 11 March 1976 In 1973 Preble and Youngner repo[r] ... See full document
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Physical map of the origin of defective DNA in herpes simplex virus type 1 DNA.
... The disappearance of the 5.4- to 5.7-Mdal EcoRI dDNA fragments after digestion with BamHI and the appearance of a new cluster of fragments, 2.6 to 3.2 Mdal, plus two 1-Mdal fragments B2 [r] ... See full document
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Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis.
... Synthesis of cRNA in MDBK cells infected with influenza WSN vifus ts mutants cRNA produced gg Virus Complementation group 2 4 6 Hours after infection 8 FIG.. Kinetics of cRNA production [r] ... See full document
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Temperature-Sensitive Mutants of Adenovirus Type 12 Defective in Viral DNA Synthesis
... The ability of the viral DNA-defective ts 401 and 406 mutants to transform hamster embryo cell cultures at th.e nonpermissive temperature was compared to the WT transformation induction.[r] ... See full document
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Cooperative transformation studies with temperature-sensitive mutants of Rous sarcoma virus.
... The present report deals with the methods used for the isolation of ts mutants from stocks of Bryan high-titer RSV of the morphr and morpht type of morphological conversion 14, with the [r] ... See full document
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