Real time reverse transcriptase PCR
Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... DNA or RNA amplification methods for detection of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but are often ...
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... multiplex real-time reverse transcriptase PCR has been developed for the rapid detection and identifica- tion of eight medically important flaviviruses from laboratory-reared, ...
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... the real-time SYBR green I and postamplification melting temper- ature curve analysis for the mD1/TS and 3 ⴕ NC protocols using identical amplification ...multiplex, real-time reverse ...
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Evaluation of Twelve Real Time Reverse Transcriptase PCR Primer Probe Sets for Detection of Pandemic Influenza A/H1N1 2009 Virus
... Real-time reverse transcriptase PCR (rRT-PCR) assays have greatly contributed to the detection, control, and prevention of the pandemic influenza A/H1N1 2009 ...
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Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... Two real-time reverse transcriptase PCR (RT-PCR) assays performed in a LightCycler instrument were developed and compared regarding specificity and sensitivity by the use of ...
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... We have developed and evaluated a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (EV-D68) in clinical specimens. This assay was ...
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Development of a pan Simbu real time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
... In order to determine the analytical sensitivity of the SBV-S3 assay, an SBV-S3 standard was generated. There- fore, the gel-purified SBV-S3 PCR fragment was li- gated into the vector pGEM-Teasy (Promega, ...
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Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... in PCR efficiency and nucleic acid quantification compared to probes that were fully complementary (35), another study has shown that a single mismatch in the probe-binding re- gion resulted in a quantification ...
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Development of a Real Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
... RRT- PCR assay versus VI include RT-PCR-inhibitory substances in the samples, the use of a one-step RT-PCR method, inefficient RNA extraction procedures, and the potential for RNA to rapidly degrade ...
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Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... a real- time RT-PCR assay (NL-N assay) that can detect the four genetic lineages of hMPV described to date with comparable ...during PCR and is thus efficiently hydrolyzed by the endonuclease ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... We report the development of three different assays and a cost-effective approach for diagnosis and virus surveillance. This method was effectively used during the dengue outbreak in Singapore in 2005. A cost-effective ...
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... multiplex real- time RT-PCR for the detection, quantitation, and differentiation of DENV-1 to -4 and CHIK in patient ...outcome. Real-time PCR has brought true quantitation into ...
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Comparison of FilmArray and Quantitative Real Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens
... qRT-PCR assays. Testing was also performed by the VSPB at the CDC using the qRT-PCR assays for the detection of NP2 and VP40 genes (10, 11). While only whole blood was evaluated with the FilmArray system, ...
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Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... It is noteworthy that the sensitivity and specificity of the cELISA when evaluated by the duplex real-time qRT-PCR assay were 65.2% (95% CI, 55.3%, 75.1%) and 97.3% (95% CI, 94.7%, 99.9%), ...
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Quantification of Substance P mRNA in Human Immune Cells by Real-Time Reverse Transcriptase PCR Assay
... The PCR primers and the MB probes used were designed with Primer Express software (PE Applied Biosys- tems) and were synthesized by Integrated DNA Technologies, ...
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Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... fourplex real-time RT-PCR assays and by the nested RT-PCR assay (Table ...fourplex real-time RT-PCR assays (Table ...
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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... The PCR primers and MB were designed based on the sequence of the CCR5 gene (GenBank accession ...the real-time RT-PCR, the mRNA of a housekeeping gene, GAPDH, was ...
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... Thus, the CSF RT-PCR assay outperformed virus isolation, which is considered the diagnostic standard for CSF (8, 24, 28). CSFV Haiti-96 was detected in the nasal swabs and tonsil scrapings of contact-infected pigs ...
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Evaluation of a Rapid and Completely Automated Real Time Reverse Transcriptase PCR Assay for Diagnosis of Enteroviral Meningitis
... CSF viral cultures lack sensitivity, rarely provide results in a clinically relevant time frame, and require high-level technical expertise to perform. Nucleic acid amplification tests (NAATs) for enterovirus RNA ...
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Human skin identification using specific gene marker at different storage temperatures
... conserved genes that encode stratum corneum proteins (Jackson et al., 2005). LCE genes, including late cornified envelope 1D (LCE1D), are abundant and highly sensitive skin biomarkers. Molecular methodologies have been ...
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