reference genes
A sequence-based approach to identify reference genes for gene expression analysis
... 15 genes were identified as the most consistently expressed across normal and cancerous lung tissue (Table ...reliable reference genes. B2 M has previously been utilized as a reference gene ...
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Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells
... stable genes based on the M values led to the identification of TBP and PGK1 as the most stable pair of reference genes, and 18S was considered as the least stable one in all groups (Figure ...2 ...
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Evaluation of Reference Genes for Differential Gene Expression Study of Bovine Tuberculosis
... stable reference gene, across the infection status (bTB positive or negative) and across experimental conditions (4 hours or overnight antigen stimulation), and is thus the reference gene of choice for use ...
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Validation of Reference Genes for mRNA Quantification in Adjuvant Arthritis
... as reference genes in DRG (18S), muscle (B2M), capsule (GAPDH/UCE), epiphysis (GAPDH) and periosteum ...as reference genes in spinal cord (PPIB/ GAPDH), DRG (ARBP/GAPDH), tendon (ACTB/B2M), ...
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Identification of suitable reference genes for hepatic microRNA quantitation
... gender to specify groups, we determined which candidates had the highest inter-group variation between male and female liver samples (Figure 2). In this figure the error bars represent the average of the intragroup ...
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Reference genes identification for normalization of qPCR under multiple stresses in Hordeum brevisubulatum
... of genes in ...stable reference genes for ...ence genes that can be used to accurately examine gene expression in subsequent research in ...11 reference gene candidates were selected ...
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Identification of stable reference genes for quantitative PCR in koalas
... Supplementary Table S3: Overall ranking of candidate reference genes analysed separately in Queensland, South Australia and both populations combined Ranking order Method.. Recommended c[r] ...
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Identification of stable reference genes for quantitative PCR in koalas
... candidate reference genes in koala lymph node tissues was analysed through Ct ...candidate reference genes expressed at a medium level, with average Ct values ranging from ...
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Selection of reference genes for qPCR in hairy root cultures of peanut
... of reference genes for RT-qPCR in hairy root ...candidate reference genes were measured in peanut hairy root cul- tures treated with two elicitors (NaOAc and ...21 genes were dis- ...
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Selection of reference genes for expression studies with fish myogenic cell cultures
... the genes were ranked in the following order from most to least stable: Hprt1>RNApolII >Ppia>Ef1 α >Sdha> Actb>18SrRNA>Pgk when all time points were ...the genes were ranked: ...
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Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood
... ence genes, often termed ''house-keeping ...ence genes can vary significantly between different tissues or conditions, thus making it impossible to use the same reference genes for various ...
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Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements.
... Recently, a growing number of published articles have reflected the importance of reference genes and the need to validate them for each particular experimental model. Most of these studies have been done ...
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Selection of reference genes for diurnal and developmental time course real time PCR expression analyses in lettuce
... ence genes the expression profile of ...stable reference genes according to geNorm results, and using target- and run-specific amplification with qBase Plus software version ...
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Evaluation of the reference genes in human adipose tissue and lipoma samples
... two genes did not change during adipogenesis, the authors of this study con- cluded that both IPO8 and FBXL10 are good candi- dates for reference genes for gene expression analy- sis in human omental ...
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Identification of Optimal Reference Genes for Examination of Gene Expression in Different Tissues of Fetal Yaks
... Reference genes are defined as genes that are stably expressed across all tissues/cell types; how- ever, no single gene is constitutively expressed in all tissues/cell types and under all ...
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Evaluation of reference genes for gene expression studies in senna (Cassia angustifolia Vahl.) using quantitative real-time polymerase chain reaction.
... the reference genes caADH, caATPsyn, caEEF1-α and caEEF1-β recorded an M value lower than GeNorm ...stable reference genes for target gene quantification in ...The reference gene ADH ...
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Genomic selection of reference genes for real-time PCR in human myocardium
... Primers for all 10 candidate reference genes were designed using Primer3 software [18] within regions spanned by the Affymetrix probe set target sequences (Table 1). At least one primer in every primer pair ...
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RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
... of genes, on which the normalization is computed, does not change across experiments; and (ii) that a single global scaling factor is valid over the entire dynamic range of ex- ...of reference genes, ...
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Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR
... candidate reference genes [glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine/ threonine-protein phosphatase catalytic subunit (PP2A), elongation factor 1 alpha (EF1-alpha), polyubiquitin (OUB2), ...
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Variation in stability of endogenous reference genes in fallopian tubes and endometrium from healthy and ectopic pregnant women
... Different Reference Genes to Normalise Data for Genes of Interest In order to test the effects of using various reference genes on the gene expression profiles, the relative expression ...
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