[PDF] Top 20 Analysis of the herpes simplex virus type 1 OriS sequence: mapping of functional domains.

... Analysis of origin sequences in herpes simplex virus type 2 HSV-2 has shown that deletion of part of a sequence corresponding to site III in HSV-1 resulted in a dramatic loss in DNA repl[r] ... See full document

... and analysis of virions for gH ...extracellular virus was inactivated with citrate buffer (pH 3.0) for 1 min, followed by three washes with ...Complemented virus was harvested 24 h after ... See full document

... In herpes simplex virus type 1 (HSV-1), one molecule of VP19C and two of VP23 form a three-pronged structure that acts to stabilize the capsid shell through interactions with ... See full document

... discern functional coding regions that are far from the norm for these ...that analysis of se- quence characteristics gives a clear positive evaluation of cod- ing potential, but a negative indication must ... See full document

... disrupted functional activity are misfolded, 1 (655AS656) folds correctly as a monomer but oligomerizes inefficiently, and 2 (331AS332 and 376AS377) fold and oligomerize ...immunofluorescence ... See full document

... transcripts, 1 ␮l of cDNA for detection of cellular transcripts, or 100 ng of dorsal root ganglion DNA for detection of viral ...Table 1 and were designed using Primer Express software ...7700 ... See full document

... Consistent with this model are the following observations: i certain late proteins e.g., VP154 can be expressed in the absence of functional VP175 this study, ii VP175 is required for ce[r] ... See full document

... the functional domains of a protein (reviewed in reference ...this type of ...genome), virus replication was inhibited, whereas inhibition was not observed in cells with 2 to 10 copies ... See full document

... The herpes simplex virus type 1 genome contains three origins of DNA replication: two copies of oriS and one copy of ...Although oriS has been characterized extensively, ... See full document

... Phosphopeptide analysis of mutant ICP4 molecules contain- ing various portions of the molecule showed at least five dif- ferent peptide profiles, with the general pattern being that smaller ICP4 proteins showed ... See full document

... acid sequence of OBPC is identical to the C-terminal 487 amino acids of OBP; however, OBPC lacks the N-terminal domain of OBP, which includes five of the six conserved heli- case motifs, the ATP-binding and ... See full document

... deletion analysis reported here confirm and extend the observations of Wong and Schaffer (80) by demonstrating that progressive loss of oriS-flanking sequences on either side of the core origin leads to a ... See full document

... this virus (vBSGFPNES) has undergone a spontaneous deletion ...that virus growth might have been inhibited by the expression of ICP27 containing the NES mu- tation, even when the virus is grown in a ... See full document

... mutant virus-infected Vero cells ...gradient analysis or electron microscopy ...ts1201 virus-infected cells indicated that cleavages may occur inside of the capsids, and only these capsids can mature ... See full document

... Figure 1 shows the physical maps of HSV-1 strain 17 and HSV-2 strain HG52 for the restriction endonucleases XbaI, HindIII, EcoRI, BglII, HpaI, KpnI, and BamHI in the region 30 to 50 map [r] ... See full document

... and functional fea- tures ...and oriS has revealed similarities between these two origins and other eukaryotic origins and has begun to provide insight into the mechanism by which HSV-1 DNA ... See full document

... Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.. Activation of immediate-early, early, and[r] ... See full document

... Incubation of infected cell extracts with the lower-affinity site II DNA as a probe resulted in the appearance of two protein-DNA complexes with mobilities identical to those of the A an[r] ... See full document

... One-step growth analysis of HSV-1 was performed in the Vps4 stable cell lines. Untreated cells or cells treated with ponA were infected with HSV-1. At various times postinfec- tion, samples were ... See full document

... The fact that all of the mutants sequenced had mutations resulting in amino acid substitutions in the gK gene strengthens the identification of this gene with the synl locus.. The fragme[r] ... See full document