[PDF] Top 20 Use of an Automated Multiple Locus, Variable Number Tandem Repeat Based Method for Rapid and High Throughput Genotyping of Staphylococcus aureus Isolates
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Use of an Automated Multiple Locus, Variable Number Tandem Repeat Based Method for Rapid and High Throughput Genotyping of Staphylococcus aureus Isolates
... Development and automation of the typing assay. Figure 1A shows that there is a linear correlation between the bacterial lysis time and the amounts of total DNA extracted. The max- imal amount of DNA was recovered from ... See full document
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Multiple Locus Variable Number Tandem Repeat Assay Analysis of Methicillin Resistant Staphylococcus aureus Strains
... a multiple-locus variable-number tandem-repeat assay (MLVA) for Staphylococcus aureus could predict pulsed-field gel electrophoresis (PFGE) types ...more ... See full document
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Microfluidic Chip Based Multiple Locus Variable Number Tandem Repeat Fingerprinting with New Primer Sets for Methicillin Resistant Staphylococcus aureus
... MLVF method that would be optimal in an outbreak ...the isolates were unrelated by both MLVA and spa ...outbreak isolates from nonoutbreak ...grouped isolates that were identified as being ... See full document
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Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable Number Tandem Repeat Analysis Typing Method
... contain variable repeated units among strains (43, 60). Systems based on a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) have been used extensively for typ- ing ... See full document
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High Throughput Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat Genotyping for Mycobacterium tuberculosis Epidemiological Studies
... loci-processed isolates, the unit costs and turnaround times for the conventional and automated methods were compared in our setting, based on a processing batch of 94 samples and 2 controls ...for ... See full document
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Rapid Variable Number Tandem Repeat Genotyping for Mycobacterium leprae Clinical Specimens
... improve throughput, we have developed a multiplex- PCR approach suitable for rapid amplification of 15 VNTR loci from ...clinical isolates from the Philippines (gene diversity of ...27 ... See full document
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Comparison of Two Multiple Locus Variable Number Tandem Repeat Analysis Methods for Molecular Strain Typing of Human Brucella melitensis Isolates from the Middle East
... MLVA method requires more time for typing from start to finish, requires less technical expertise, and involves less-expensive materials, although it does require BioNumerics software, which is quite costly and ... See full document
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Multiple Locus Variable Number Tandem Repeat Analysis as a Tool for Subtyping Listeria monocytogenes Strains
... MLVA method for ...selected based on their ability to subtype primarily serotypes 1/2a, 1/2b, and ...small repeat units of 6 bp to 15 bp. The MLVA is a high-throughput, rapid ... See full document
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Comparison of Multiple Locus Variable Number Tandem Repeat Analysis with Pulsed Field Gel Electrophoresis, spa Typing, and Multilocus Sequence Typing for Clonal Characterization of Staphylococcus aureus Isolates
... Staphylococcus aureus, especially methicillin-resistant ...increasing number of nosocomial and community-acquired ...S. aureus isolates, which allows monitoring of local outbreaks and ... See full document
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Multiple Locus Variable Number Tandem Repeat Analysis for Typing of Staphylococcus epidermidis
... of Staphylococcus epidermidis is in- creasingly ...nosocomial isolates of ...easy-to-use, rapid typing methods with high ...The method has high discriminatory power but is ... See full document
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Improved Multiple Locus Variable Number Tandem Repeat Assay for Staphylococcus aureus Genotyping, Providing a Highly Informative Technique Together with Strong Phylogenetic Value
... S. aureus in a method called multilocus variable-number tandem- repeat fingerprinting (MLVF) to reflect the fact that the data are not interpreted in terms of repeat copy ... See full document
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Novel Multiple Locus Variable Number Tandem Repeat Analysis Method for Rapid Molecular Typing of Human Staphylococcus aureus
... standard method for the typing of Staphylococcus aureus isolates ...a method based on the unique lengths of the intergenic regions contain- ing repetitive DNA loci (5, 8, 9, 12, ... See full document
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Multiple Locus Variable Number Tandem Repeat Analysis of Salmonella enterica Serovar Typhi
... the most variable VNTR loci for epidemiological typing. Forty- three potential VNTRs were identified using the web-based resource tool (6) (see Table S1 in the supplemental material). The primers were ... See full document
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Development and validation of a multiple locus variable number tandem repeat analysis (MLVA) scheme for Fusobacterium necrophorum
... Seventy-three tandem repeat regions (Table S1) were identified from the whole genome shotgun sequence of ...Accession number AJSY00000000.1) using the Tandem Repeats Finder software ...34 ... See full document
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Mycobacterium bovis population structure in cattle and local badgers: co-localisation and variation by farm type
... of multiple, distal land parcels. The use of land parcel locations may provide a more accurate and precise indication of true herd locations and quantify the opportunity for herds to interact spatially with ... See full document
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Francisella tularensis Strain Typing Using Multiple Locus, Variable Number Tandem Repeat Analysis
... The tularemia cases represented by the California isolates group into two separate clusters (I and II), suggesting the presence of a very subdivided reservoir of F. tularensis biovars in this region. Temporal ... See full document
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Multiple Locus Variable Number Tandem Repeat Analysis of the Swine Dysentery Pathogen, Brachyspira hyodysenteriae
... discriminatory method for strain ...a multiple-locus variable-number tandem-repeat analysis (MLVA) method that could be used in basic veterinary diagnostic ... See full document
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High Genetic Diversity Revealed by Variable Number Tandem Repeat Genotyping and Analysis of hsp65 Gene Polymorphism in a Large Collection of “Mycobacterium canettii” Strains Indicates that the M tuberculosis Complex Is a Recently Emerged Clone of “M canettii”
... 2003. Genotyping by multiple-locus variable-number tandem repeat analysis shows that all the strains belong to a single but very distant group when compared to strains of ... See full document
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Clostridium botulinum Group I Strain Genotyping by 15 Locus Multilocus Variable Number Tandem Repeat Analysis
... Microbial Tandem Repeats database (http: ...accession number NC_009698), 57 potential VNTR loci were detected, including 10 pre- viously investigated by Macdonald and colleagues ... See full document
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Development of Multiple Locus Variable Number Tandem Repeat Analysis for Molecular Typing of Mycoplasma pneumoniae
... the number of TRs, PCR primers targeting the regions flanking TR loci were ...clinical isolates, 5 type 2 clinical isolates, and 1 isolate that could not be classified by P1 gene PCR-RFLP ...Each ... See full document
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