[PDF] Top 20 Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.

... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document

... after origin DNA unwinding or after unwinding and primer synthesis ...T-antigen binding to Pol/Prim in primer synthesis and elongation (14, 15, 23–25, 30, 31, 69, 72, 77), but interference with ... See full document

... (Ad) DNA-binding protein (DBP) is essential for the elongation phase of Ad DNA replication by unwinding the template in an ATP-independent fashion, employing its capacity to form ...Ad ... See full document

... As shown in Fig. 8A, the T-ag OBD spiral hexamer is con- figured such that many of the A1 and B2 residues that interact preferentially with ssDNA in the NMR studies (Fig. 2) are exposed in the vicinity of the gap, ... See full document

... Footprint and fragment assays led to the following conclusions: i in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A prote[r] ... See full document

... Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location 10, 11, 12, 14, the bound protein can be localized at 0.7 i 0.05 on the SV40 D[r] ... See full document

... LEWTON,1 ROBERT TJIAN,2 AND PETER TEGTMEYERl* Received 4 October 1982/Accepted 10 January 1983 Investigation of the DNA binding properties of the simian virus 40 SV40 A protein large T a[r] ... See full document

... single-stranded DNA binding and the ability to cause structure distortion of origin DNA, suggesting that single- stranded DNA binding is also needed for structural ...from ... See full document

... detect binding of full-length T antigen to fluorescent probes under the same salt ...increase binding of T antigen to the probe, we performed binding isotherms at lower salt concen- trations, either ... See full document

... analyzed binding to larger subfragments of the core ...duplex DNA was provided by experiments conducted with a 17-bp oligo- nucleotide containing site ... See full document

... DNA binding studies showed that subunits of the simian virus 40 A protein T antigen bound to each of the four recognition pentanucleotides in the origin palindrome but did so with reduce[r] ... See full document

... for DNA unwinding, we generated mutant 31-bp DNAs with a single base-pair substitution at each site within the central ...31-bp origin DNA unwinding and binding assays of wild-type and mutant ... See full document

... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...The replication of simian virus ... See full document

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document

... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document

... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document

... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document

... The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited footprint assay or excess fragment assay amounts of DNase I.. At l[r] ... See full document

... Simian virus 40 SV40 large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication.. Molecular cloning: a laboratory manual, 2nd ed.[r] ... See full document

... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document