[PDF] Top 20 Characterization of a temperature-sensitive mutant of human adenovirus type 7.
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Characterization of a temperature-sensitive mutant of human adenovirus type 7.
... To determine if the viral polypeptides were assembled into capsomeres, cytoplasmic extracts of WT- and mutant-infected cells labeled with [35S]methionine were analyzed on sucrose gradien[r] ... See full document
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Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.
... The localization of the p14 and p32 proteins to wild-type VV has recently been described (42). The peripheral membrane protein p14 (gene A27L) is probably involved in the infection process (31–33) as well as in ... See full document
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Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.
... nonpermissive temperature for an enzymatic step required between virus penetration and proviral DNA ...ts mutant indicates that the conditional phenotype is manifested at a time after proviral integration ... See full document
5
Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature sensitive mutant
... 1. Temperature-sensitive (ts) defect of Smu1 alters the nuclear localization of SRSF2, ALY, and ...ts mutant tsTM18 cells were grown at 34˚C or incubated at 39˚C for 8 hours and ...and mutant ... See full document
7
Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1.
... In this series of experiments, replicate 25-cm2 cultures of Vero cells were infected in duplicate with the parent, mutant, or rescued virus 5 PFU per cell and incubated in a 34°C incubat[r] ... See full document
7
Isolation and preliminary characterization of a phosphonoacetic acid-resistant and temperature-sensitive mutant of herpes simplex virus type 1.
... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document
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Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
... The present study demonstrates that a molecular form of T antigen induced by ts 900, a transformation-defective ts mutant of SV40 29, is more heat labile than that induced by WT virus, i[r] ... See full document
6
Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus.
... In the present growth of wild-type RS virus, ts-1, ts-2, and ts-7 incubated for 24 h at permissive or restrictive study this was confirmed when it was shown temperature that at least 13 [r] ... See full document
5
Preliminary characterization of a temperature-sensitive mutant of Moloney murine leukemia virus that produces particles at the restrictive temperature.
... The infectivity of ts7, determined by a focus-forming unit assay, was reduced at least 100-fold when the virus was assayed at the nonpermissive temperature 39°C as compared with assay at[r] ... See full document
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Characterization of a temperature-sensitive, hexon transport mutant of type 5 adenovirus.
... In the infected cells, hexons are produced in vast excess of the amount found in viral particles 643 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Infection of KB cel[r] ... See full document
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Isolation and characterization of temperature-sensitive mutants of adenovirus type 7.
... Intertypic complementation tests with temperature-sensitive mutants of adenovirus type 5 showed that the mutant in complementation group A failed to complement H5ts125 a DNA-binding prot[r] ... See full document
7
Characterization of a Temperature-Sensitive Fiber Mutant of Type 5 Adenovirus and Effect of the Mutation on Virion Assembly
... 6A, radioactivity was detected in particles having the electron microscopic appearance and the buoyant density of empty capsids p 1.29 g/ml and complete virions p 1.33 g/ml 30, 40.. The [r] ... See full document
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Genetic reconstitution of the human Adenovirus type 2 temperature sensitive 1 mutant defective in endosomal escape
... 51 human serotypes have been identified to-date, and classified into 6 species ...An adenovirus mutant, Ad2-ts1 had been isolated upon chemical mutagenesis in the past, and shown to have unprocessed ... See full document
7
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... the human growth hormone, this mutagenesis approach resulted in the identifi- cation of residues involved in hormone binding ...IN mutant of the 24 constructed ...HIV-1 mutant mt4 described in this ... See full document
8
Cryo-Electron Microscopy Structure of Adenovirus Type 2 Temperature-Sensitive Mutant 1 Reveals Insight into the Cell Entry Defect
... Vertex release may require a conformational change that is blocked in Ad2ts1. A conformational change in the vertex re- gion has been proposed after the interaction of Ad with inte- grins on the cell surface (14). ... See full document
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Selection and Preliminary Characterization of Temperature-Sensitive Mutants of Type 5 Adenovirus
... Preliminary biochemical and immunological characterization of the mutants indicated that: i the single mutant in complementation group I did not replicate its deoxyribonucleic acid DNA o[r] ... See full document
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Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants
... It can be seen that ts 3 fails to synthesize any of the capsid antigens; ts 11, ts 4, and ts 7 representing three distinct complementation groups produced normal precipitin lines corresp[r] ... See full document
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Genetic analysis of adenovirus type 2. I. Isolation and genetic characterization of temperature-sensitive mutants.
... DISCUSSION The present investigation describes the isolation of temperature-sensitive mutants of human Ad2, their division into 13 complementation groups, and the construction of a provi[r] ... See full document
7
Characterization of a host range mutant of human adenovirus 12 defective in early region 1B.
... 132 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We isolated an adenovirus 12 early region 1B mutant in2O6B by ligation of the cleaved DNA-protein complex and transf[r] ... See full document
5
Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5.
... SHARP Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and MRC Institute of Virology, Glasgow Gll 5JR, Scotlan[r] ... See full document
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