[PDF] Top 20 Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... the virus. However, in comparison with conventional RT-PCR, the fluorogenic assay presents many advantages, the most important being a closed system in which the tube is never opened post amplification, ... See full document
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Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura virus
... the virus. However, in comparison with conventional RT-PCR, the fluorogenic assay presents many advantages, the most important being a closed system in which the tube is never opened post amplification, ... See full document
7
Development of a Highly Sensitive Genus Specific Quantitative Reverse Transcriptase Real Time PCR Assay for Detection and Quantitation of Plasmodium by Amplifying RNA and DNA of the 18S rRNA Genes
... of PCR assays in detection of Plasmodium at the genus or species level is commonly described in para- sites/ l or percent ...(real-time PCR, molecular beacon, nucleic acid sequence- based ... See full document
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Development of One Step, Real Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
... TaqMan-based, real-time quantitative reverse transcriptase PCR (qRT-PCR) for the rapid detection and quantitation of human coronaviruses (HCoVs) OC43 and ...allows ... See full document
5
Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... RT- PCR assay we have developed can be used for epidemiological studies of the EV-D68 outbreak and for virus monitoring in sub- sequent ...this time whether typing EV-D68 will be useful for patient ... See full document
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... template quantitation from samples containing a large range of concentrations can be accom- plished without the need to repeat the assay using a diluted sam- ... See full document
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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
... several virus species exist together in the field, detection and diagnosis of WYMV is an important area of ...are time-consuming and have low levels of sensitivity and ...the virus titre in local ... See full document
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... to virus isolation, which produced 73 possibly test- positive scores, the single-dye NS5 assay had 39 ...by one additional passage in C6/36 cell culture and used for RNA extraction, heterologous ... See full document
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Development and Evaluation of One Step TaqMan Real Time Reverse Transcription PCR Assays Targeting Nucleoprotein, Matrix, and Hemagglutinin Genes of Equine Influenza Virus
... TaqMan real-time reverse transcription-PCR (rRT-PCR) assays by the use of the minor groove binding probe to detect a wide range of equine influenza virus (EIV) strains comprising ... See full document
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Development of a Real Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
... RRT- PCR assay versus VI include RT-PCR-inhibitory substances in the samples, the use of a one-step RT-PCR method, inefficient RNA extraction procedures, and the potential for RNA to ... See full document
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Evaluation of Twelve Real Time Reverse Transcriptase PCR Primer Probe Sets for Detection of Pandemic Influenza A/H1N1 2009 Virus
... 2009 virus rRT-PCR assays. Twelve rRT-PCR assays for pandemic influenza A/H1N1 2009 virus were selected from work pub- lished between July 2009 and July ...of PCR cycles was set at 45, ... See full document
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Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... TaqMan real-time RT-PCR, it has the advantages of simplicity in primer design and universal RT- PCR protocols suitable for multiple target ...the real-time RT-PCR protocol ... See full document
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Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... four real-time PCR assays that presented discrepant results, defined as a sample that exhibited a positive result in one assay but a negative result in another assay, were arbitrarily chosen ... See full document
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... the development of three different assays and a cost-effective approach for diagnosis and virus ...the virus in clinical ...the PCR equipment used ...in one PCR run than in a ... See full document
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... the development of a real-time, multiplex RT-PCR to detect various flaviviruses has been demonstrated in this study for the detection of YFV, WNV, JEV, SLEV, and DENV-1 to -4 in ... See full document
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Development of an Internally Controlled Real Time Reverse Transcriptase PCR Assay for Pan Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays
... and evaluation of the pan-DENV assay, which is a species-specific internally controlled rRT-PCR for pan-DENV ...of time during which a confirmed molecular diagnosis of DENV infection can be ... See full document
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The development of a rapid SYBR one step real time RT PCR for detection of porcine reproductive and respiratory syndrome virus
... of virus in cell cultures is technically diffi- cult and time-consuming and thus is not suitable for rou- tine diagnostic ...several reverse transcription-PCR (RT-PCR) methods have been ... See full document
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High Throughput Real Time Reverse Transcription PCR Quantitation of Hepatitis C Virus RNA
... RESULTS The concentration of HCV RNA in the first reference stan- dard was calculated from its specific activity measured on a scintillation counter. The number of RNA molecules synthe- sized was 10 9 copies per ml. ... See full document
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... (TaqMan)–reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected ... See full document
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Real-time PCR for mrna quantitation
... a real-time PCR experiment, from experimental planning and setup through data analysis and graphical presentation ...complex real-time PCR experiments, having an organized and ... See full document
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