[PDF] Top 20 Isolation and localization of herpes simplex virus type 1 mRNA.

... Finally, vii the 4-kb species, as well as a range of smaller species of viral RNA species found hybridizing to HindIII fragment G cellulose and blots, are shown in the short unique regio[r] ... See full document

... Location of abundant early HSV-1 mRNA' Size RNA RNA Major restriction fragNen to RN bands hybridizing Restriction fragment band hybridizing to 3' end probe of 2 h p.i.. b The sizes shown[r] ... See full document

... a virus specifying this insertion was easily isolated and displayed rep- lication properties similar to those of the wild-type ...wild- type parental KOS genome by homologous recombination methods ... See full document

... the herpes- viral glycoproteins gD and gH (11, 79, ...normal localization of ...the localization of gM (data not ...subcellular localization when expressed in isolation or in the ... See full document

... Understanding of the many complex roles of Hsp90 has been aided by the discovery and development of small molecules that specifically target and inhibit the Hsp90 chaperone ma- chinery. Geldanamycin is a naturally ... See full document

... tumor virus oncoproteins, adenovirus E1A and SV40 TAg, accumulate within or adjacent to ND10 ...the localization of the E7 oncoprotein in relation to ND10 has not been examined, it is attached to the ... See full document

... HSV- 1-infected Vero cells and in transfected cells expressing only this viral gene ...intracellular localization of the ...Golgi localization pattern, we examined whether the UL51 protein ... See full document

... To detect viral antigens in optic nerve tissue, the optic nerve, optic chiasm, and optic tract were harvested as a unit and separated from the retina. Tissues were homogenized in lysis buffer (0.2 M Tris-Cl [pH 7.2], 10 ... See full document

... We isolated specific HSV-1 mRNA species mapping within the region of interest by hybridization of late polyA mRNA to the appropriate restriction fragment bound to cellulose see above.. S[r] ... See full document

... events that are required to convert nuclear primary transcripts into functional mRNA molecules in the cytoplasm. It has been recognized for some time that ICP27 can activate or repress the expression of reporter ... See full document

... picked up and were carefully subcultured into fresh petriplates containing Nutrient agar medium. The isolate was identified using molecular 16S rRNA sequencing. An isolated bacterial colony was picked and suspended in ... See full document

... the localization of capsids with respect to replication compartments, the same Vero cells infected with KOS, the UL6 mutant (hr74), or the UL32 mutant (hr64) were also labeled with the ICP8 poly- clonal antibody ... See full document

... Finally, we used deletional analysis to demonstrate that no more than 29 bases of DNA sequence 5' of the mRNA cap site are required for promoter activity in either location; this result [r] ... See full document

... SDS-PAGE analysis of extracts of Vero or n-33 cells infected at a multiplicity of infection of 20 PFU per cell with hr259, hr79, or HSV-2 186 and labeled with [35S]methionine from 3 to 1[r] ... See full document

... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document

... We have isolated mutants in a new complementation group whose mutations map to the left of alkaline nuclease within the EcoRI D fragment of HSV-1 strain KOS coordinates 0.086 to 0.194.. [r] ... See full document

... MOORE,1 AND LARS HAARR`6 Department of Biochemistryl* and the Gade Institute, Departments of Pathology2 and Microbiology,6 University of Bergen, Bergen, Norway; Medical Research Council [r] ... See full document

... Effects of ICP0 and ICP27 on the intracellular localization of ICP4 in transient expression assays. In light of the obser- vations described above, we next asked whether in transient assays, ICP27 or ICP0 was able ... See full document

... NTAP-UL28 virus (vFH476). These fragments were not detected with a UL28-null virus (GCB), the C-terminally truncated UL28 virus (vFH499), or the UL28 (vFH505) and UL15 (vFH506 and vFH507) domain ... See full document

... of herpes simplex virus type 1 (HSV-1) gene expression during lytic infection and reactivation from viral ...of mRNA, mRNA stability, and levels of protein ... See full document