[PDF] Top 20 Mapping early transcripts of herpes simplex virus type 1 by electron microscopy.

... Regions of the genome which are infrequently R-looped by early polyribosomal RNA were revealed by i analysis of the R-loop frequency distribution of a randomly arranged population of int[r] ... See full document

... (Fig. 1), and quantitated by phosphorimaging. Values from the virus dilutions were then compared with the protein standard curve to obtain the amount of protein (in nanograms) per ...each virus, and ... See full document

... ICP0 transcripts not only in the TG and the SCG, which were positive by ISH, but also in other latently infected struc- tures such as the spinal cord and the ...ICP0 transcripts is due to the slow ... See full document

... of herpes simplex virus type 1 (HSV-1), recent evi- dence suggests that the association of viral genomes with ND10 may be functionally important, because genomes that are so ... See full document

... FIG. 2. (A) Photograph of a longitudinal cryosection through the rostral half of a trigeminal ganglion of a mouse killed 2 days after injection. The section was immunostained with a polyclonal antiserum tagged with ... See full document

... ICP0 mRNA levels are suppressed in a neuronal cell line that expresses the LATs. Since HSV-1 replication is dependent on the expression of the viral IE genes, we hypothesized that the LATs may reduce HSV-1 ... See full document

... taining transcripts. The distribution of IE110 transcripts in cells infected with the mutant virus 110C1, in which both in- trons of IE110 have been precisely removed, indicates that exonic sequences ... See full document

... Other links between the EJC, splicing, and translation have recently been uncovered. A protein termed eIF4AIII, which is highly similar to translation initiation factors eIFAI and eIFAII, has been shown to be an integral ... See full document

... Electron microscopy. The purity of the in vitro-produced capsids was analyzed by negative staining. Succinctly, purified capsids were adsorbed on hexagonal 200-mesh copper grids coated with Formvar and ... See full document

... than virus-expressed gM ...and virus-expressed gM to nuclear mem- branes in a process that required at least one other viral ...The early gM nuclear pool does not transit through the ... See full document

... 2169 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 H[r] ... See full document

... When the cranial cavities of these mice were opened, hydrocephali had developed in the group of mice that received whole immune spleen cells and those treated with anti-immunoglobulin pl[r] ... See full document

... HEp-2 cells were infected with HSV-1(F) at 5.0 PFU per cell and at 16.5 h after infection were either Epon embedded and processed conventionally or high-pressure frozen and freeze substituted. In both cases, ... See full document

... 3281 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest During latent herpes simplex virus type 1 HSV-1 infection in the trigeminal ganglia of mice, three virus-specific t[r] ... See full document

... demonstrate that in human trigeminal ganglia, the smaller (1.35 kb) HSV-1 transcript differs from the larger (1.85 kb) LAT by excision of an intron near its 5' end; they are otherwise colinear, and 5' coterminal. ... See full document

... For infectivity assay a 1.5-ml sample of infected cell suspension was frozen, thawed, and then sonicated for 1 min at 10 kcycles/s with a 727 Downloaded from http://jvi.asm.org/ on Novem[r] ... See full document

... TEVETHIA* Department of Microbiology and Cancer Research Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 A functions involved in the altered su[r] ... See full document

... It should be added that RNase treatment of a comparable sample of heat-denatured HSV DNA which had been purified in cesium sulfate gradient resulted in a significant decrease of [3Hjurid[r] ... See full document

... Finally, vii the 4-kb species, as well as a range of smaller species of viral RNA species found hybridizing to HindIII fragment G cellulose and blots, are shown in the short unique regio[r] ... See full document

... Preliminary immunoprecipitation experiments indicated that the target antigen was a glycoprotein of about 59,000 molecular weight, and reaction of the antibody with intertypic recombinan[r] ... See full document