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[PDF] Top 20 Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)

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Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)

Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)

... validated reference or internal control genes have not been reported in eggplant which could pose a limitation for undertaking gene expression studies in this important ... See full document

11

Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures.

Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures.

... cyclophilin genes, in two rice genotypes cultivated in low temperature (13°C) conditions in vegetative stage ...the expression stability of the candidate reference ...indicated genes for all ... See full document

15

Validation of reference genes for expression analysis by quantitative real time PCR in Leptinotarsa decemlineata (Say)

Validation of reference genes for expression analysis by quantitative real time PCR in Leptinotarsa decemlineata (Say)

... the reference genes in ...validated reference genes could also serve as the basis for selection of candidate internal controls in any given insect ... See full document

10

Cloning and validation of reference genes for normalization of gene expression studies in pearl millet [Pennisetum glaucum (L.) R. Br.] by quantitative real-time PCR

Cloning and validation of reference genes for normalization of gene expression studies in pearl millet [Pennisetum glaucum (L.) R. Br.] by quantitative real-time PCR

... alleviating genes that provide stress adaptation to this ...in gene discovery, characterization, transcript pro filing, and their application in crop improvement ...programs. Gene expression ... See full document

8

Selection of reliable reference genes for quantitative real time PCR in human T cells and neutrophils

Selection of reliable reference genes for quantitative real time PCR in human T cells and neutrophils

... systematic validation of reference gene stability has thus far been lacking for either model, ...the expression stability of potential reference genes in unstimulated and ... See full document

11

Genomic selection of reference genes for real-time PCR in human myocardium

Genomic selection of reference genes for real-time PCR in human myocardium

... because expression of both genes tended to be lower in failed myocardium compared with non-failed myocar- dium, TPT1 and EEF1A1 require further validation in a larger sample to confirm that their ... See full document

12

Validation of internal reference genes for quantitative real time PCR in a non model organism, the yellow necked mouse, Apodemus flavicollis

Validation of internal reference genes for quantitative real time PCR in a non model organism, the yellow necked mouse, Apodemus flavicollis

... Background: Reference genes are used as internal standards to normalize mRNA abundance in quantitative real-time PCR and thereby allow a direct comparison between ...these ... See full document

7

Selection of reference genes for quantitative real time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

Selection of reference genes for quantitative real time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

... level. Quantitative qRT-PCR is the most extensively used method for validating genome-wide gene expression analyses, but in order to obtain reliable results, suitable reference ... See full document

10

Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR

Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR

... the expression stability of the eight reference genes in each culti- var ...stable reference genes which is consistent with the analysis performed across the three cultivars to- ... See full document

12

Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)

Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)

... qRT-PCR reference genes in several teak ...of reference genes for gene expression assessment in leaves, flowers, seedlings, roots, and lignified stem and branch secondary ... See full document

14

Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization

Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization

... expressed genes as normalizers has crucial role in accurate and reliable expression analysis estimated by quantitative real-time polymerase chain reaction ...Recent studies have ... See full document

13

Validation of reference genes for quantitative real time PCR (qPCR) analysis of Actinobacillus suis

Validation of reference genes for quantitative real time PCR (qPCR) analysis of Actinobacillus suis

... no studies have been done to characterise refer- ence genes for ...preliminary studies sug- gested that reference genes used to study closely related organisms were not ...eight ... See full document

8

Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures

Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures

... Background: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression ...multiple reference genes has become the most popular ... See full document

8

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real time quantitative RT PCR (RT qPCR)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real time quantitative RT PCR (RT qPCR)

... We confirmed the amplification of specific RT-qPCR products by performing a melting-curve step at the end of each run. Serial dilution curves for each primer allowed us to calculate RT-qPCR efficiencies. The 10-fold ... See full document

12

Identification and validation of quantitative real time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L f )

Identification and validation of quantitative real time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L f )

... qRT-PCR reference genes in several teak ...of reference genes for gene expression assessment in leaves, flowers, seedlings, roots, and lignified stem and branch secondary ... See full document

13

Reference gene selection for quantitative PCR studies in bovine neutrophils

Reference gene selection for quantitative PCR studies in bovine neutrophils

... analysis. Expression stability of the remaining genes was evaluated using four software programs: geNorm, NormFinder, BestKeeper, and the compara- tive delta Cq ...neutrophil reference genes, ... See full document

9

Selection of reference genes for diurnal and developmental time course real time PCR expression analyses in lettuce

Selection of reference genes for diurnal and developmental time course real time PCR expression analyses in lettuce

... For each candidate reference gene, a blast search was carried out in the expressed sequence tag (EST) Compositae Genome Project Database (CGPDB 2013; http://cgpdb.ucdavis.edu/) against L. sativa coding DNA ... See full document

10

Validation of housekeeping genes for normalizing RNA expression in real-time PCR

Validation of housekeeping genes for normalizing RNA expression in real-time PCR

... different time points, which included cells challenged with TB anti- ...Our selection of subjects and time points mirrored experimental protocols that we propose to use in our laboratory to study ... See full document

6

Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

... 14 genes were less than ...candidate reference genes were sta- ble. As one of the least stable genes, the instability of APT has been reported in papaya under six experimental con- ditions ... See full document

17

Quantitative biomarker analysis of synovial gene expression by real time PCR

Quantitative biomarker analysis of synovial gene expression by real time PCR

... each gene of interest while simultaneously correcting for differences in reverse tran- scription ...the genes relevant to inflammatory synovitis ...in PCR amplification, a significant cause of assay ... See full document

9

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