[PDF] Top 20 The a sequence is dispensable for isomerization of the herpes simplex virus type 1 genome.

... The herpes simplex virus type 1 (HSV-1) genome consists of two components, L (long) and S (short), that invert relative to each other during productive infection to ... See full document

... The overwhelming evidence in support of this conclusion includes a description of a sequence-independent genome isomerization 12, the characterization of genome recombination events that[r] ... See full document

... one genome equivalent and ...The isomerization mechanism has been previously linked to the en- capsidation process, as the alternative cleavage of concatemeric molecules is part of the mechanism leading to ... See full document

... 2169 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 H[r] ... See full document

... Analysis of gB translated in vitro in the presence and absence of membranes showed that i gB is inserted into membranes and glycosylated cotranslationally; ii a large portion of the gB p[r] ... See full document

... Table 1) confirmed and extended our previous obser- vations made using the amplicon assay ...of virus progeny, c1, c3, and c4 (substitutions of the pac2 GC element, unconserved region, and consensus se- ... See full document

... HSV genome and a mutant HSV genome that has a single SpeI recognition site deleted ...wild-type virus genome ...(lanes 1 and 4) or twice (lane ...pW4.2 genome but lacking ... See full document

... (Fig. 1) and the 400-bp regions with large numbers of polymorphic sites in 6 of the 8 genes, exclud- ing UL28 and ...out sequence anal- ysis of these regions for the other 31 strains (Table ... See full document

... In the experiments we report here, labeled DNA fragments were used as probes to monitor the purification of these factors, to determine the specificity of binding of the viral proteins r[r] ... See full document

... Considerably higher amplification of this sequence was observed in defective viral DNA: up to 14 copies were detected to be inserted in the repeat units of a major class of defective her[r] ... See full document

... viral genome copy number might influence reactiva- tion from the latent state can be ...viral genome prior to sufficient immediate-early gene transcription (13, ...viral genome copy number had not ... See full document

... We isolated specific HSV-1 mRNA species mapping within the region of interest by hybridization of late polyA mRNA to the appropriate restriction fragment bound to cellulose see above.. S[r] ... See full document

... MOORE,1 AND LARS HAARR`6 Department of Biochemistryl* and the Gade Institute, Departments of Pathology2 and Microbiology,6 University of Bergen, Bergen, Norway; Medical Research Council [r] ... See full document

... If the recombination event that gave rise to the inversion fragment occurred by cleavage-ligation at the position of site-specific cleavage directed by pacl case 1, then the 5'-flanking [r] ... See full document

... Short, duplicated sequence indicative of the recombinogenicity of the junction between a unique and an inverted repeat sequence in the S component of the herpes simplex virus type 1 geno[r] ... See full document

... ICP27 is an RNA-binding protein that shuttles between the nucleus and cytoplasm (1, 23–25, 32, 39, 50, 51): it is essential for HSV replication and one function appears to involve the export of some HSV late RNAs ... See full document

... The predicted amino acid homology for the proteins encoded between the potential alternate splice acceptor sites of HSV-1 suggests that there may be alternate splicing patterns in the EB[r] ... See full document

... The studies mentioned above showed complex patterns of mRNA hybridizing to several regions 749 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have examined in detai[r] ... See full document

... a sequence was sufficient for viability and represents the first demonstra- tion that the minimum packaging signal, Uc-DR1-Ub, is func- tional in the context of a full-length HSV-1 ...control virus ... See full document

... Restriction endonucleases were purchased from Takara Shuzo Company Kyoto, Japan and Toyobo Company Osaka, Japan, and conditions for digestion were those recommended by the 1877 Downloade[r] ... See full document