[PDF] Top 20 Validation of reference genes for quantitative real time PCR (qPCR) analysis of Actinobacillus suis
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Validation of reference genes for quantitative real time PCR (qPCR) analysis of Actinobacillus suis
... ence genes for A. suis and our preliminary studies sug- gested that reference genes used to study closely related organisms were not ...eight reference genes was assessed in ... See full document
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Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization
... expressed genes as normalizers has crucial role in accurate and reliable expression analysis estimated by quantitative real-time polymerase chain reaction ...housekeeping genes ... See full document
13
Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR
... candidate reference genes at different developmental stages of the olive fruit across three different olive culti- vars independently and together, in an attempt to iden- tify most suitable reference ... See full document
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Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures
... Background: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression ...multiple reference genes has become the most popular ...of ... See full document
8
Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in Plutella xylostella (Lepidoptera: Plutellidae)
... systematic validation of ref- erences genes in qRT-PCR studies, normalization procedures have received little attention for this spe- cies, with many studies continuing to use a single ... See full document
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Selection of reliable reference genes for quantitative real time PCR in human T cells and neutrophils
... systematic validation of reference gene stability has thus far been lacking for either model, ...potential reference genes in unstimulated and anti-CD3/CD28 activated T cells and in unstimu- ... See full document
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Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real time PCR
... Rafaela M Paim 1 , Marcos H Pereira 1,3 , Raffaello Di Ponzio 1 , Juliana O Rodrigues 2 , Alessandra A Guarneri 2,3 , Nelder F Gontijo 1,3 and Ricardo N Araújo 1,3* Abstract Background: Rhodnius prolixus is a ... See full document
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Microarray based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress
... Six genes that presented high levels of stability in the microarray were tested in RT-qPCR, as well as two other genes commonly used in RT-qPCR data ...possible reference gene combinations: act + ... See full document
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Genomic selection of reference genes for real-time PCR in human myocardium
... both genes tended to be lower in failed myocardium compared with non-failed myocar- dium, TPT1 and EEF1A1 require further validation in a larger sample to confirm that their expression levels do not differ ... See full document
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Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures.
... of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data ... See full document
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Identification of stable reference genes for quantitative PCR in koalas
... Quantitative real time PCR has been used widely in molecular biology research to evaluate gene expression under certain ...a reference gene is ...control genes is critical as ... See full document
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Validation of reference genes for expression analysis by quantitative real time PCR in Leptinotarsa decemlineata (Say)
... house-keeping genes (Actin, ACT1 and ACT2; ADP- ribosylation factor, ARF1 and ARF4; TATA box binding protein, TBP1 and TBP2; ribosomal protein RP4 and RP18; translation elongation factor 1 α EF1 α ) was evaluated ... See full document
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Establishing reference genes for use in real-time quantitative PCR analysis of early equine embryos
... for normalizing gene expression data for morula to expanded blastocyst stage in vivo equine 428. embryos; this should assist in studies to examine expression of genes potentially invo[r] ... See full document
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... potential reference genes for qRT-PCR in the Cactaceae ...typical reference genes were selected to determine the most stable refer- ence genes for qRT-PCR normalization in ... See full document
12
Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)
... qRT-PCR reference genes in several teak ...of reference genes for gene expression assessment in leaves, flowers, seedlings, roots, and lignified stem and branch secondary xylem of ... See full document
14
Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)
... 14 genes were less than ...candidate reference genes were sta- ble. As one of the least stable genes, the instability of APT has been reported in papaya under six experimental con- ditions ... See full document
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Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)
... validated reference or internal control genes have not been reported in eggplant which could pose a limitation for undertaking gene expression studies in this important ...control genes from eggplant ... See full document
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Identification and validation of quantitative real time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L f )
... qRT-PCR reference genes in several teak ...of reference genes for gene expression assessment in leaves, flowers, seedlings, roots, and lignified stem and branch secondary xylem of ... See full document
13
Validation of internal reference genes for quantitative real time PCR in a non model organism, the yellow necked mouse, Apodemus flavicollis
... Background: Reference genes are used as internal standards to normalize mRNA abundance in quantitative real-time PCR and thereby allow a direct comparison between ...potential ... See full document
7
Sequencing and validation of housekeeping genes for quantitative real time PCR during the gonadotrophic cycle of Diploptera punctata
... possible reference genes were selected based on their traditional use and stability described in reported validation studies in other insects ...eight genes described in this study, one other ... See full document
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