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[PDF] Top 20 Functional limits of oriP, the Epstein-Barr virus plasmid origin of replication.

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Functional limits of oriP, the Epstein-Barr virus plasmid origin of replication.

Functional limits of oriP, the Epstein-Barr virus plasmid origin of replication.

... Deletions that left six or fewer 30-bp repeats in an EBV plasmid or plasmids reconstructed with up to five copies of a 30-bp repeat oligonucleotide were defective for plasmid maintenance[r] ... See full document

10

The Latent Origin of Replication of Epstein-Barr Virus Directs Viral Genomes to Active Regions of the Nucleus

The Latent Origin of Replication of Epstein-Barr Virus Directs Viral Genomes to Active Regions of the Nucleus

... at oriP and parts of different EBV genomes (10, ...latent origin is specifically enriched with his- tone modifications that correlate with active transcription such as histone H3 and H4 acetylation as well ... See full document

14

Replacement of the Epstein-Barr Virus Plasmid with the EBER Plasmid in Burkitt's Lymphoma Cells

Replacement of the Epstein-Barr Virus Plasmid with the EBER Plasmid in Burkitt's Lymphoma Cells

... Epstein-Barr virus (EBV) is present in tumor cells of more than 90% of cases of Burkitt’s lymphoma (BL) in areas of endemicity in equatorial Africa and New Guinea, as well as in tumor cells of about ... See full document

6

Rep*: a Viral Element That Can Partially Replace the Origin of Plasmid DNA Synthesis of Epstein-Barr Virus

Rep*: a Viral Element That Can Partially Replace the Origin of Plasmid DNA Synthesis of Epstein-Barr Virus

... include oriP-BamHI C-Luc (29); DS-BamHI C-Luc (29); FR-BamHI C-Luc, which was derived from oriP-BamHI C-Luc by an EcoRV-HpaI deletion which removes the DS of oriP; FR-D-Luc, which was derived from ... See full document

10

Identification of EBNA1 amino acid sequences required for the interaction of the functional elements of the Epstein-Barr virus latent origin of DNA replication.

Identification of EBNA1 amino acid sequences required for the interaction of the functional elements of the Epstein-Barr virus latent origin of DNA replication.

... Unlike the interaction of bEBNA1 with onP, in which one element FR is bound prior to interaction Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest a bEBNAl proteins 1.4 pm[r] ... See full document

9

Replication from oriP of Epstein-Barr Virus Requires Exact Spacing of Two Bound Dimers of EBNA1 Which Bend DNA

Replication from oriP of Epstein-Barr Virus Requires Exact Spacing of Two Bound Dimers of EBNA1 Which Bend DNA

... Some replication activity that is independent of EBNA1 has been attributed to oriP (2, 18), although such activity appears to be very inefficient compared to EBNA1- dependent replication (18, ... See full document

9

Evidence for DNA Hairpin Recognition by Zta at the Epstein-Barr Virus Origin of Lytic Replication

Evidence for DNA Hairpin Recognition by Zta at the Epstein-Barr Virus Origin of Lytic Replication

... the replication deficiency of Zta mutants was an indirect consequence of an inability to activate Rta expression, which is also essential for viral DNA replica- tion, we cotransfected an Rta expression ... See full document

10

Epstein Barr Nuclear Antigen 1 modulates replication of oriP plasmids by impeding replication and transcription fork migration through the family of repeats

Epstein Barr Nuclear Antigen 1 modulates replication of oriP plasmids by impeding replication and transcription fork migration through the family of repeats

... sites limits repli- cation from oriP in a manner consistent with it impeding the migration of replication ...impedes replication fork migration is dependent upon the number of binding sites in ... See full document

15

Epstein-Barr Virus Episome Stability Is Coupled to a Delay in Replication Timing

Epstein-Barr Virus Episome Stability Is Coupled to a Delay in Replication Timing

... the replication timing and chromatin organiza- tion of ...early replication origins ...that replication timing was important for genome stability during S phase (12, ...early replication ... See full document

9

Essential Role of Rta in Lytic DNA Replication of Epstein-Barr Virus

Essential Role of Rta in Lytic DNA Replication of Epstein-Barr Virus

... the origin of lytic ...a functional gene for ZEBRA, were transfected with empty vector (cytomegalovirus [CMV]) or expression vectors encoding Z(S186A) or wt ZEBRA in the absence and presence of ...EBV ... See full document

16

oriP is essential for EBNA gene promoter activity in Epstein-Barr virus-immortalized lymphoblastoid cell lines.

oriP is essential for EBNA gene promoter activity in Epstein-Barr virus-immortalized lymphoblastoid cell lines.

... on oriP for Cp and Wp activity in our assay system reflected the plasmid maintenance function of oriP, in conjunction with EBNA 1, rather than its enhancer ...lacking oriP might reflect loss ... See full document

11

Regulation of Epstein-Barr Virus Origin of Plasmid Replication (OriP) by the S-Phase Checkpoint Kinase Chk2

Regulation of Epstein-Barr Virus Origin of Plasmid Replication (OriP) by the S-Phase Checkpoint Kinase Chk2

... B replication timing ...the replication timing of ...inhibits OriP DNA replication and plasmid ...with OriP plasmid-coexpressing EBNA1 and assayed for transient DNA ... See full document

9

Multiple EBNA1-binding sites are required to form an EBNA1-dependent enhancer and to activate a minimal replicative origin within oriP of Epstein-Barr virus.

Multiple EBNA1-binding sites are required to form an EBNA1-dependent enhancer and to activate a minimal replicative origin within oriP of Epstein-Barr virus.

... However, the requirements for EBNA1-binding sites for enhancer function parallel the requirements for activation of replication: seven to nine 30-bp repeats are required for full activit[r] ... See full document

10

New Noncoding Lytic Transcripts Derived from the Epstein-Barr Virus Latency Origin of Replication, oriP, Are Hyperedited, Bind the Paraspeckle Protein, NONO/p54nrb, and Support Viral Lytic Transcription

New Noncoding Lytic Transcripts Derived from the Epstein-Barr Virus Latency Origin of Replication, oriP, Are Hyperedited, Bind the Paraspeckle Protein, NONO/p54nrb, and Support Viral Lytic Transcription

... the virus performs during its infection ...simplex virus that are ex- pressed during the lytic cycle ...of virus gene expression and DNA replication to virion packaging, processing, and ... See full document

13

Identification of cellular factors that bind specifically to the Epstein-Barr virus origin of DNA replication.

Identification of cellular factors that bind specifically to the Epstein-Barr virus origin of DNA replication.

... To identify cellular proteins possibly involved in EBV plasmid maintenance, protein-DNA gel retardation experiments and DNase I footprinting were performed, * using HeLa cell nuclear ext[r] ... See full document

6

Cellular proteins bind to the downstream component of the lytic origin of DNA replication of Epstein-Barr virus.

Cellular proteins bind to the downstream component of the lytic origin of DNA replication of Epstein-Barr virus.

... 7. Functional analysis of mutant oriLyt plasmids in the transient replication ...(the replication efficiency of wild-type plasmid ...transient replication assay as described elsewhere ... See full document

9

Specific Methylation Patterns in Two Control Regions of Epstein-Barr Virus Latency: the LMP-1-Coding Upstream Regulatory Region and an Origin of DNA Replication (oriP)

Specific Methylation Patterns in Two Control Regions of Epstein-Barr Virus Latency: the LMP-1-Coding Upstream Regulatory Region and an Origin of DNA Replication (oriP)

... The Epstein-Barr virus (EBV) can establish at least four different forms of latent ...the origin of replication (oriP) region and the latent membrane protein 1 (LMP-1) regulatory ... See full document

6

Plasmid-like replicative intermediates of the Epstein-Barr virus lytic origin of DNA replication.

Plasmid-like replicative intermediates of the Epstein-Barr virus lytic origin of DNA replication.

... to plasmid progeny ...the oriP-oriLyt vector p562 were analyzed on two-dimensional agarose gels (top) and detected after Southern blot ...II) plasmid DNA during the first (no intercalating dye) and ... See full document

9

EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.

EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.

... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ... See full document

7

Regulation of Epstein-Barr Virus OriP Replication by Poly(ADP-Ribose) Polymerase 1

Regulation of Epstein-Barr Virus OriP Replication by Poly(ADP-Ribose) Polymerase 1

... (lytic origin of replication), GCCCGT TGGGTTTCATTAAG and ...a plasmid expressing shRNA against PARP1 (shPARP1) or an empty vector as a negative control (shControl) as described ... See full document

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