[PDF] Top 20 Isolation and characterization of herpes simplex virus mutants containing engineered mutations at the DNA polymerase locus.
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Isolation and characterization of herpes simplex virus mutants containing engineered mutations at the DNA polymerase locus.
... Virus AB10 formed plaques on Vero cells as well as on DP6 cells Table 2, demonstrating that the 144-bp fragment containing the small open reading frame is not essential for virus growth [r] ... See full document
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Effects of Exonuclease Activity and Nucleotide Selectivity of the Herpes Simplex Virus DNA Polymerase on the Fidelity of DNA Replication In Vivo
... pHOS1 DNA was extracted by the cetyltrimethylammonium bromide DNA precipitation method ...of mutations, each mutant plasmid was directly analyzed by sequencing the SupF gene, using the fmol ... See full document
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Fine mapping and molecular cloning of mutations in the herpes simplex virus DNA polymerase locus.
... 236 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Mutations in five phenotypically distinct mutants derived from herpes simplex virus type 1 strain KOS which lie in o[r] ... See full document
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Site-Directed Mutagenesis of Large DNA Palindromes: Construction and In Vitro Characterization of Herpes Simplex Virus Type 1 Mutants Containing Point Mutations That Eliminate the oriL or oriS Initiation Function
... automated DNA sequencing proce- ...tical mutations into both copies of oriS in the viral ...published virus lacking both copies of oriS described to date (R7711) (29) was constructed using a complex ... See full document
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Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.
... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document
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Effects of mutations in the Exo III motif of the herpes simplex virus DNA polymerase gene on enzyme activities, viral replication, and replication fidelity.
... III mutants for pol function in tran- sient transfection ...gene containing mutations at codons 441 and 443 (DE35) were included as positive and negative controls, ...progeny virus was ... See full document
8
Mutations That Increase DNA Binding by the Processivity Factor of Herpes Simplex Virus Affect Virus Production and DNA Replication Fidelity
... the herpes simplex virus processivity factor UL42 with the catalytic subunit of the viral polymerase (Pol) and DNA are critical for viral DNA ...of DNA binding in vitro, ... See full document
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Isolation and Characterization of the Herpes Simplex Virus 1 Terminase Complex
... NTAP-UL28 virus (vFH476). These fragments were not detected with a UL28-null virus (GCB), the C-terminally truncated UL28 virus (vFH499), or the UL28 (vFH505) and UL15 (vFH506 and vFH507) domain ... See full document
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Isolation and characterization of herpes simplex virus type 1 host range mutants defective in viral DNA synthesis.
... We have isolated mutants in a new complementation group whose mutations map to the left of alkaline nuclease within the EcoRI D fragment of HSV-1 strain KOS coordinates 0.086 to 0.194.. [r] ... See full document
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Sensitivity of arabinosyladenine-resistant mutants of herpes simplex virus to other antiviral drugs and mapping of drug hypersensitivity mutations to the DNA polymerase locus.
... AraA and arabinosylthymine araT are adenosine and thymidine analogs, Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this report, we examine seven HSV mutants known [r] ... See full document
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Processing of Lagging-Strand Intermediates In Vitro by Herpes Simplex Virus Type 1 DNA Polymerase
... active DNA replication were identified by the incorporation of 5⬘ bromo-2-deoxyuridine ...Viral DNA replication compart- ments in nontransfected or transfected cells were detected by the immunolocal- ... See full document
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Herpes Simplex Virus ICP0 Mutants Are Hypersensitive to Interferon
... ICP0 mutants revealed above stems at least in part from a failure to accumulate adequate quantities of IE ...ICP0 mutants (1, ...infecting virus, although in1814, n212, and ...ICP0 mutants ... See full document
5
Identification and Characterization of a Herpes Simplex Virus Gene Product Required for Encapsidation of Virus DNA
... In this study, we report the characterization of an HSV-1 mutant that has a temperature-sensitive processing defect in the nucleocapsid structural polypeptide p40 and fails to encapsidat[r] ... See full document
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Mutations that specifically impair the DNA binding activity of the herpes simplex virus protein UL42.
... and DNA by virtue of its ability to bind both the catalytic subunit and the DNA ...such DNA was greater than that of either protein alone (18, ...replicative DNA polymerase d in ...the ... See full document
7
Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein.
... In the absence of viral DNA replication, HSV Pol colocalized with the HSV DNA-binding protein ICP8 in nuclear framework-associated structures called prereplicative sites.. In the presenc[r] ... See full document
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Acyclovir-resistant mutants of herpes simplex virus type 1 express altered DNA polymerase or reduced acyclovir phosphorylating activities.
... BWr, a new acyclovir-resistant mutant derived from the Patton strain of herpes simplex virus type 1, induced a DNA polymerase resistant to inhibition by acyclovir triphosphate, but, unli[r] ... See full document
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Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document
13
Assessing the contribution of the herpes simplex virus DNA polymerase to spontaneous mutations
... sperm DNA using Lipofectamine rea- gent according to the manufacturer's recommendations ...and virus inoculated at an MOI = 5 in 2 mL volume for 1 ...the DNA was precipitated, centrifuged at 14,000 ... See full document
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Herpes simplex virus DNA polymerase as the site of phosphonoacetate sensitivity: temperature-sensitive mutants.
... POWELL* Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030 Received for publication 9 June 1977 Temperature-sensitive ts mutants in a surprisingly [r] ... See full document
8
DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.
... The remaining mutants studied, all of which were DNA- at 39 C Table 1, fell into three categories with respect to their ability to induce DP activity at 39 C Table 2: i mutants belonging[r] ... See full document
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