[PDF] Top 20 Multilocus Sequence Typing Scheme for Enterococcus faecium
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Multilocus Sequence Typing Scheme for Enterococcus faecium
... (glucose-6-phosphate dehydrogenase), purK (phosphoribosylaminoimidazol car- boxylase ATPase subunit), and pstS (phosphate ATP-binding cassette transport- er). Fragments were amplified from bacterial lysates by PCR with ... See full document
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Core Genome Multilocus Sequence Typing Scheme for High Resolution Typing of Enterococcus faecium
... Whole-genome sequencing and assembly. Genomic DNA was iso- lated from cell pellets using a DNeasy blood and tissue kit (Qiagen, Co- penhagen, Denmark); for the isolates from Denmark, the Wizard genomic DNA purification ... See full document
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Development and Application of a Multilocus Sequence Typing Scheme for Streptococcus gallolyticus subsp gallolyticus
... Each cycle sequencing reaction mixture was prepared with the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems, Darmstadt, Germany). Excess dye terminators and primers were removed by centrif- ugation with ... See full document
7
Multilocus Sequence Typing Scheme versus Pulsed Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates
... multispacer sequence typing (MST) (20) and multi- locus sequence analysis (MLSA) (21) confirmed the existence of three principal groups within ...gene sequence analysis ... See full document
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Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii
... a typing system is required that is characterized by excellent discriminatory power but even more importantly by high inter- laboratory reproducibility that permits the development of a central databank of strain ... See full document
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The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus
... The current epidemiological understanding of D. nodosus shows it is ubiquitous within the global sheep population (Kennan et al., 2014), with relatively new presence confirmed in Brazil (Aguiar et al., 2011) and ... See full document
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Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Genome Wide Typing of Clostridium difficile
... reference sequence among all strains included; any addition of strains would result in novel SNV results, which could lead to conflicting results, as SNV typing does not rely on a fixed nomenclature but is ... See full document
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Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole Genome Sequence Based Typing of Listeria monocytogenes
... MLST typing, a prerequisite for successful interlaboratory ex- change of ...gene typing (spa) and now contains ⬎ 300,000 typing entries originating from ⬎ 100 countries, might serve as a blueprint ... See full document
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Multilocus Sequence Typing Analysis of Human and Animal Clostridium difficile Isolates of Various Toxigenic Types
... A multilocus sequence typing (MLST) scheme was developed to study the genetic relationships and popu- lation structure of 72 Clostridium difficile isolates from various hosts, geographic ... See full document
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Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System with Pulsed Field Gel Electrophoresis for Molecular Typing of Vancomycin Resistant Enterococcus faecium
... Relationship of Rep-PCR types and antimicrobial suscepti- bility. The relationships of Rep-PCR types and antimicrobial susceptibility patterns are shown in Table 1 and in the supple- mental material. Thirty-eight of the ... See full document
5
Multilocus Variable Number Tandem Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing
... A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of ...scribed multilocus sequence typing scheme for the ... See full document
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Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... the sequence vari- ation of fewer loci might be adequate for subspecies ...applied sequence typing to one or two genes and successfully differen- tiated most isolates of ... See full document
9
Multilocus Sequence Typing of Mycobacterium xenopi
... mycobacterial typing, and RFLP can be used to differentiate ...MLST scheme, it becomes possible to routinely examine the distribution, evolution, and population structure of ... See full document
5
Multilocus Sequence Typing of Clostridium difficile
... high-throughput multilocus sequence typing (MLST) scheme for Clostridium difficile was developed and validated using a diverse collection of 50 reference isolates representing 45 different PCR ... See full document
9
Characterization of Epidemiologically Unrelated Acinetobacter baumannii Isolates from Four Continents by Use of Multilocus Sequence Typing, Pulsed Field Gel Electrophoresis, and Sequence Based Typing of blaOXA 51 like Genes
... The present work revealed that the tree topologies for all identified alleles of the seven genes were inconsistent, with the trees for gyrB and gpi being incongruent in relation to each other and to the trees for the ... See full document
8
Development of a multilocus sequence typing scheme for the molecular typing of Mycoplasma pneumoniae
... Description of Mycoplasma pneumoniae strains used in this study, their sequence type ST and allelic profile, and their MLVA and P1 types.. Strains isolated from the same patient are indi[r] ... See full document
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Multilocus Sequence Typing Scheme for Enterococcus faecalis Reveals Hospital Adapted Genetic Complexes in a Background of High Rates of Recombination
... A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus ...different ... See full document
9
Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... molecular typing methods, PFGE and REA, had already been developed to study intraspecific variations in ...the typing system will assign a different type to two unrelated strains randomly sampled in the ... See full document
8
Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
... Few typing methods have been able to detect signif- icant differences between strains, including one previous attempt to subtype ...MLST scheme developed in this study was able to discriminate between ... See full document
9
A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis
... cgMLST scheme to describe the ...cgMLST scheme was also tested to investigate the relatedness of strains putatively involved in hospital outbreak events and to obtain further insights into ...cgMLST ... See full document
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